Zinc-dependent regulation of zinc import and export genes by Zur

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487083.do
DOI
10.1038/ncomms15812
Title
Zinc-dependent regulation of zinc import and export genes by Zur
Description
We are indebted to Drs Yann Dufour and Tim Donohue (UW-Madison) forproviding microarrays and assistance in bioinformatics analysis. Wethank E.-J. Woo for help in refining model structures. This work wassupported by a grant (2014R1A2A1A01002846) from the Ministry of Science,ICT and Future Planning and a grant (2011-0031960) for IntelligentSynthetic Biology Center of Global Frontier Project to J.-H.R.; S.-H.C.was supported by BK21-Plus fellowship for graduate students forBiological Sciences at SNU.
abstract
In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression. At the same time, Zur represses genes for zinc uptake. At micromolar zinc concentrations (phase II), oligomeric Zur binding with footprint expansion upward from the Zur box results in high zitB induction. Our findings reveal a mode of zinc-dependent gene activation that uses a single metalloregulator to control genes for both uptake and export over a wide range of zinc concentrations.
provenance
Made available in Cube on 2018-09-28T16:24:48Z (GMT). No. of bitstreams: 0
language
English
author
Choi, Seung-Hwan
Lee, Kang-Lok
Shin, Jung-Ho
Cho, Yoo-Bok
Cha, Sun-Shin
Roe, Jung-Hye
accessioned
2018-09-28T16:24:48Z
available
2018-09-28T16:24:48Z
issued
2017
citation
NATURE COMMUNICATIONS(8)
issn
2041-1723
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487083.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273977
Jurisdiction
Rep.of Korea
Project Name
Advanced Training Program for Biological Sciences
rights
openAccess
type
article


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