Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasingcellular association

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487082.do
DOI
10.5483/BMBRep.2017.50.2.150
Title
Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasingcellular association
Description
This work was supported by the Industrial Technology Innovation program(10063322, Development of Animal Cell Culture based hEC- SOD forManagement of Atopic Dermatitis) funded By the Ministry of Trade,industry & Energy.
abstract
Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that Zn2+ promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of Zn2+. This resulted in increased inhibitory effects of rhSOD3 on NF-kB and STAT3 signals in the presence of Zn2+, which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine.
provenance
Made available in Cube on 2018-09-28T16:24:46Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Younghwa
Jeon, Yoon-Jae
Ryu, Kang
Kim, Tae-Yoon
accessioned
2018-09-28T16:24:46Z
available
2018-09-28T16:24:46Z
issued
2017
citation
BMB REPORTS(50): 2
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487082.do
Funder
산업통상자원부
Funding Program
바이오산업핵심기술개발
Project ID
1415151512
Jurisdiction
Rep.of Korea
Project Name
Development of Animal Cell Culture based hEC-SOD for Management of Atopic Dermatitis
rights
openAccess
subject
Anti-inflammatory
Cytopermeability
Heparin Binding Domain
Superoxidedismutase 3
Zinc (II) ion
type
article


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