Zinc finger protein 143 expression is closely related to tumormalignancy via regulating cell motility in breast cancer

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487080.do
DOI
10.5483/BMBRep.2017.50.12.177
Title
Zinc finger protein 143 expression is closely related to tumormalignancy via regulating cell motility in breast cancer
Description
This work was supported in part by National Cancer Center GrantNCC-1710180 (to HJ YOU) and National Research Foundation of Korea Grantfunded by the Korea Government (MSIP, South Korea) (No.2015R1A2A2A01003829).
abstract
We previously reported the involvement of zinc-finger protein 143 (ZNF143) on cancer cell motility in colon cancer cells. Here, ZNF143 was further characterized in breast cancer. Immunohistochemistry was used to determine the expression of ZNF143 in normal tissues and in tissues from metastatic breast cancer at various stages. Notably, ZNF143 was selectively expressed in duct and gland epithelium of normal breast tissues, which decreased when the tissue became malignant. To determine the molecular mechanism how ZNF143 affects breast cancer progression, it was knocked down by infecting benign breast cancer cells with short-hairpin (sh) RNA-lentiviral particles against ZNF143 (MCF7 sh-ZNF143). MCF7 sh-ZNF143 cells showed different cell-cell contacts and actin filament (F-actin) structures when compared with MCF7 sh-Control cells. In migration and invasion assays, ZNF143 knockdown induced increased cellular motility in breast carcinoma cells. This was reduced by the recovery of ZNF143 expression. Taken together, these results suggest that ZNF143 expression contributes to breast cancer progression.
provenance
Made available in Cube on 2018-09-28T16:24:43Z (GMT). No. of bitstreams: 0
language
English
author
Paek, A. Rome
Mun, Ji Young
Hong, Kyeong-Man
Lee, Jongkeun
Hong, Dong Wan
You, Hye Jin
accessioned
2018-09-28T16:24:43Z
available
2018-09-28T16:24:43Z
issued
2017
citation
BMB REPORTS(50): 12
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487080.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274416
Jurisdiction
Rep.of Korea
Project Name
Graduate and professional program for senior healthcare industry
rights
openAccess
subject
Invasive ductal carcinoma
Metastasis
Motility
ZNF143
type
article


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