Zebrafish as an animal model in epilepsy studies with multichannel EEGrecordings

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487077.do
DOI
10.1038/s41598-017-03482-6
Title
Zebrafish as an animal model in epilepsy studies with multichannel EEGrecordings
Description
We thank Dr. Christophe Bernard at the Institut de Neurosciences desSystemes for constructive discussions and suggestions. This research wassupported by grants from the Basic Science Research Program of theNational Research Foundation (NRF-2014R1A1A3050285) and DGIST MIREBraiNProgram (2016010043) funded by the Ministry of Science, ICT & FuturePlanning and the Chonnam National University Hospital BiomedicalResearch Institute (CRI14071-3).
abstract
Despite recent interest in using zebrafish in human disease studies, sparked by their economics, fecundity, easy handling, and homologies to humans, the electrophysiological tools or methods for zebrafish are still inaccessible. Although zebrafish exhibit more significant larval-adult duality than any other animal, most electrophysiological studies using zebrafish are biased by using larvae these days. The results of larval studies not only differ from those conducted with adults but also are unable to delicately manage electroencephalographic montages due to their small size. Hence, we enabled non-invasive long-term multichannel electroencephalographic recording on adult zebrafish using custom-designed electrodes and perfusion system. First, we exploited demonstration of long-term recording on pentylenetetrazole-induced seizure models, and the results were quantified. Second, we studied skin-electrode impedance, which is crucial to the quality of signals. Then, seizure propagations and gender differences in adult zebrafish were exhibited for the first time. Our results provide a new pathway for future neuroscience research using zebrafish by overcoming the challenges for aquatic organisms such as precision, serviceability, and continuous water seepage.
provenance
Made available in Cube on 2018-09-28T16:24:39Z (GMT). No. of bitstreams: 0
language
English
author
Cho, Sung-Joon
Byun, Donghak
Nam, Tai-Seung
Choi, Seok-Yong
Lee, Byung-Geun
Kim, Myeong-Kyu
Kim, Sohee
accessioned
2018-09-28T16:24:39Z
available
2018-09-28T16:24:39Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487077.do
Funder
미래창조과학부
Funding Program
개인연구지원
Project ID
1711041720
Jurisdiction
Rep.of Korea
Project Name
Development of EEG and EMG sensors for zebrafish models
rights
openAccess
type
article


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