Wogonin suppresses stem cell-like traits of CD133 positive osteosarcomacell via inhibiting matrix metallopeptidase-9 expression

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487046.do
DOI
10.1186/s12906-017-1788-y
Title
Wogonin suppresses stem cell-like traits of CD133 positive osteosarcomacell via inhibiting matrix metallopeptidase-9 expression
Description
This research is supported by the Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education (2016R1A6A1A03012862).
abstract
Background: Several efforts have been deployed to cure osteosarcoma, a high-grade malignant bone tumour in children and adolescents. However, some challenges such as drug resistance, relapse, and tumour metastasis remain owing to the existence of cancer stem cells (CSC). There is an urgent need to develop cost-effective and safe therapies.
Methods: Wogonin, an extract from the root of Scutellaria baicalensis, has long been considered as a promising natural and safe compound for anti-tumourigenesis, particularly to inhibit tumour invasion and metastasis. Hoechst 33,342 staining, wound healing assay, sphere formation assay, western blotting, and gelatin zymography assays were performed in CD133 positive osteosarcoma cell.
Results: In this study, we examined the effect of Wogonin on the mobility of human osteosarcoma CSC. Wogonin induces apoptosis of human osteosarcoma CSC, inhibits its mobility in vitro via downregulation of MMP-9 expression, and represses its renewal ability.
Conclusions: We demonstrated that Wogonin decreases the renewal capacity of CSC. By inhibiting the formation of and reducing the size of spheres, Wogonin at a concentration of 40-80 mu M effectively minimizes potential risk from CSC. Taken together, we have demonstrated a new approach for developing a potential therapy for osteosarcoma.
provenance
Made available in Cube on 2018-09-28T16:23:49Z (GMT). No. of bitstreams: 0
language
English
author
Do Luong Huynh
Kwon, Taeho
Zhang, Jiao Jiao
Sharma, Neelesh
Gera, Meeta
Ghosh, Mrinmoy
Kim, Nameun
Cho, Somi Kim
Lee, Dong Sun
Park, Yang Ho
Jeong, Dong Kee
accessioned
2018-09-28T16:23:49Z
available
2018-09-28T16:23:49Z
issued
2017
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(17)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487046.do
Funder
교육부
Funding Program
이공학학술연구기반구축
Project ID
1345259512
Jurisdiction
Rep.of Korea
Project Name
Development and application of subtropical bioresource
rights
openAccess
subject
Wogonin
Scutellaria baicalensis
Osteosarcoma
Cancer stem cells
Mobility
type
article


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