WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing viamiRNA-dependent and -independent mechanisms

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/487033.do
DOI
10.1093/nar/gkx307
Title
WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing viamiRNA-dependent and -independent mechanisms
Description
Basic Science Research Program [2014R1A2A1A1105198 8]; Nuclear Researchand Development Program [2012-M2B2B1-2012055637]; Medical ResearchCenter (MRC) through the National Research Foundation (NRF) funded bythe Korean government (MSIP) [201409392]; NIA-IRP, NIH (to S.D., D.D.,J.L.M., M.G.) [Z01-AG000511-19]. Funding for open access charge: BasicScience Research Program through the National Research Foundation (NRF)funded by the Korean government (MSIP) [2014R1A2A1A11051988].
abstract
RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1-AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunoprecipitation of WIG1 and detection of WIG1-associated proteins using liquid chromatography-tandem mass spectrometry. We also identified WIG1-binding motifs using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation analyses. Altogether, our data indicate that WIG1 governs the miRNA-dependent and the miRNA-independent recruitment of AGO2 to lower the stability of and suppress the translation of ACOT7 mRNA.
provenance
Made available in Cube on 2018-09-28T16:23:28Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Hyung Chul
Jung, Seung Hee
Hwang, Hyun Jung
Kang, Donghee
De, Supriyo
Dudekula, Dawood B.
Martindale, Jennifer L.
Park, Byungkyu
Park, Seung Kuk
Lee, Eun Kyung
Lee, Jeong-Hwa
Jeong, Sunjoo
Han, Kyungsook
Park, Heon Joo
Ko, Young-Gyu
Gorospe, Myriam
Lee, Jae-Seon
accessioned
2018-09-28T16:23:28Z
available
2018-09-28T16:23:28Z
issued
2017
citation
NUCLEIC ACIDS RESEARCH(45): 11
issn
0305-1048
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/487033.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274430
Jurisdiction
Rep.of Korea
Project Name
BK21 PLUS Convergence HealthCare Science Program
rights
openAccess
type
article


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