Vulpinic acid contributes to the cytotoxicity of Pulveroboletusravenelii to human cancer cells by inducing apoptosis

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486953.do
DOI
10.1039/c7ra05059c
Title
Vulpinic acid contributes to the cytotoxicity of Pulveroboletusravenelii to human cancer cells by inducing apoptosis
Description
This research was supported by the Basic Science Research Programthrough the National Research Foundation of Korea (NRF), which is fundedby the Ministry of Science, ICT, & Future Planning(2015R1C1A1A02037383), and by the Ministry of Education(NRF-2012R1A5A2A28671860). Seul Lee was supported by the NRF-2015-GlobalPhD Fellowship Program of the National Research Foundation of Korea(NRF), which is funded by the Korean Government (NRF-2015H1A2A1034135).
abstract
Pulveroboletus ravenelii (Berk. et Curt.) Murr. (Boletaceae), commonly known as Ravenel's bolete, is an edible mushroom that is widely distributed in Korea, Japan, and China. P. ravenelii is used in traditional Chinese medicine and also to create color dyes. In this study, we explored the pharmacological activity of the fruiting bodies of P. ravenelii and found that their MeOH extracts showed cytotoxic activity in vitro against a variety of human cancer cells, including four lung adenocarcinoma cell lines (A549, NCI-H1264, NCI-H1299, and Calu-6), two pancreatic ductal adenocarcinoma cell lines (PANC-1 and MIA PaCa-2), and a hepatocellular carcinoma cell line (Hep G2), by inducing apoptosis. Based on the bioactivity-guided fractionation to identify the fraction with the highest cytotoxic activity, chemical investigation of the MeOH extract of the fruiting bodies of P. ravenelii led to the isolation of vulpinic acid (VA) (1) as the main component of the most cytotoxic fraction, the hexane-soluble fraction. Treatment with VA significantly reduced cell viability by inducing apoptotic cell death in all human cancer cell lines tested, with IC50 values ranging from 21.65 to 146.17 mu M. These findings provide experimental evidence for a novel biological activity of P. ravenelii against human cancer cells. We also identified VA as the main constituent that contributes to the cytotoxic activity of P. ravenelii, thus raising the possibility of using P. ravenelii as a functional food as well as a source of lead compounds for the development of novel drugs for cancer prevention and management.
provenance
Made available in Cube on 2018-09-28T16:21:20Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Sil
So, Hae Min
Roh, Hyun-Soo
Kim, Jin
Yu, Jae Sik
Lee, Seul
Seok, Soonja
Pang, Changhyun
Baek, Kwan-Hyuck
Kim, Ki Hyun
orcid
Kim, Ki Hyun/0000-0002-5285-9138; Baek, Kwan-Hyuck/0000-0002-8535-6211
accessioned
2018-09-28T16:21:20Z
available
2018-09-28T16:21:20Z
issued
2017
citation
RSC ADVANCES(7): 56
issn
2046-2069
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486953.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274065
Jurisdiction
Rep.of Korea
Project Name
21st Century Biomedical Sciences Leader Development Program
rights
openAccess
type
article


Files in This Item

There are no attached files.