Vitamin D Insufficiency Exacerbates Adipose Tissue MacrophageInfiltration and Decreases AMPK/SIRT1 Activity in Obese Rats

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486933.do
DOI
10.3390/nu9040338
Title
Vitamin D Insufficiency Exacerbates Adipose Tissue MacrophageInfiltration and Decreases AMPK/SIRT1 Activity in Obese Rats
Description
This research was supported by the Basic Science Research Programthrough the National Research Foundation of Korea (NRF) funded by theMinistry of Science, ICT and Future Planning (2014R1A1A3050953) andBrain Korea 21 Plus (22A20130012143).
abstract
Obesity is recognized as a state of chronic low-grade systemic inflammation due to adipose tissue macrophage infiltration and production of proinflammatory adipokines. Decreased vitamin D status is associated with obesity. The specific aim of the present study is to investigate the effects of vitamin D on obesity-induced adipose tissue inflammation. Male Sprague-Dawley rats were randomized and fed a normal diet (NOR, 1000 IU vitamin D/kg diet), a 45% high-fat diet (HF, 1000 IU vitamin D/kg diet), or a 45% high-fat diet containing 25 IU vitamin D/kg diet (HF+LVD) for 12 weeks. The vitamin D-insufficient diet (HF+LVD) led to vitamin D inadequacy as determined by serum 25(OH) D level, 68.56 +/- 7w.97 nmol/L. The HF+LVD group exacerbated HF-increased adipocyte size, adipogenic gene expression of PPAR gamma, adipose tissue macrophage recruitment, and proinflammatory cytokine IL-6 and TNF alpha levels in epididymal white adipose tissue. In addition, vitamin D insufficiency significantly decreased mRNA levels of beta-oxidation-related genes such as CPT1 alpha, PGC1 alpha, PPAR alpha, VLCAD, LCAD, MCAD, and UCP1. Moreover, significant decrements of SIRT1 and AMPK activity were noted in obese rats fed with a vitamin D-insufficient diet. The observed deleterious effects of vitamin D insufficiency on adipose tissue expansion, immune cell infiltration and inflammatory status suggest vitamin D plays a beneficial role in adipocyte metabolic metabolism and obesity progression. SIRT1 and AMPK activity may play a role in the mechanism of vitamin D action.
provenance
Made available in Cube on 2018-09-28T16:20:48Z (GMT). No. of bitstreams: 0
language
English
author
Chang, Eugene
Kim, Yangha
orcid
Kim, Yangha/0000-0002-7280-7597
accessioned
2018-09-28T16:20:48Z
available
2018-09-28T16:20:48Z
issued
2017
citation
NUTRIENTS(9): 4
issn
2072-6643
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486933.do
Funder
미래창조과학부
Funding Program
개인연구지원
Project ID
1711041717
Jurisdiction
Rep.of Korea
Project Name
Effects of vitamin D on obesity by the improvement of mitochondria biogenesis and function
rights
openAccess
subject
adenosine monophosphate-activated protein kinase (AMPK)
adipose tissuemacrophage infiltration
obesity
sirtulin 1 (SIRT1)
vitamin D
type
article


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