Visualization of a Mammalian Mitochondrion by Coherent X-ray DiffractiveImaging

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486920.do
DOI
10.1038/s41598-017-01833-x
Title
Visualization of a Mammalian Mitochondrion by Coherent X-ray DiffractiveImaging
Description
We acknowledge Y.S. Jeon's group for the help in the preparation ofmitochondria specimens. This work was supported by the National ResearchFoundation of Korea (NRF) grant funded by the Korean government (MSIP)through SRC (NRF-2015R1A5A1009962) and 2016R1A6B2A02005471,NRF-2015M2A2A4A03044653, and NRF-2015M2A2A6A03044907. Y. Kimacknowledges NRF-2011-Fostering Core Leaders of the Future Basic ScienceProgram (Global Ph.D. Fellowship).
abstract
We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm(3). Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.
provenance
Made available in Cube on 2018-09-28T16:20:27Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Yoonhee
Kim, Chan
Kwon, Ou Young
Nam, Daewoong
Kim, Sang Soo
Park, Jae Hyun
Kim, Sunam
Gallagher-Jones, Marcus
Kohmura, Yoshiki
Ishikawa, Tetsuya
Song, Changyong
Tae, Giyoong
Noh, Do Young
orcid
Gallagher-Jones, Marcus/0000-0003-4227-917X
accessioned
2018-09-28T16:20:27Z
available
2018-09-28T16:20:27Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486920.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273970
Jurisdiction
Rep.of Korea
Project Name
POSTECH BK21PLUS Physics Division
rights
openAccess
type
article


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