Ursodeoxycholic Acid Attenuates Endoplasmic Reticulum Stress-RelatedRetinal Pericyte Loss in Streptozotocin-Induced Diabetic Mice

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486771.do
DOI
10.1155/2017/1763292
Title
Ursodeoxycholic Acid Attenuates Endoplasmic Reticulum Stress-RelatedRetinal Pericyte Loss in Streptozotocin-Induced Diabetic Mice
Description
This study was presented as a poster at the 2015 Annual Meeting of theAssociation for Research in Vision and Ophthalmology (ARVO), Denver,Colorado, May 3-7, 2015. This study was supported by grants from theMinistry of Science, ICT and Future Planning, Republic of Korea(NRF-2013R1A2A2A010168457).
abstract
Loss of pericytes, an early hallmark of diabetic retinopathy (DR), results in breakdown of the blood-retinal barrier. Endoplasmic reticulum (ER) stress may be involved in this process. The purpose of this study was to examine the effects of ursodeoxycholic acid (UDCA), a known ameliorator of ER stress, on pericyte loss in DR of streptozotocin-(STZ-) induced diabetic mice. To assess the extent of DR, the integrity of retinal vessels and density of retinal capillaries in STZ- induced diabetic mice were evaluated. Additionally, induction of ER stress and the unfolded protein response (UPR) were assessed in diabetic mice and human retinal pericytes exposed to advanced glycation end products (AGE) or modified low-density lipoprotein (mLDL). Fluorescein dye leakage during angiography and retinal capillary density were improved in UDCA-treated diabetic mice, compared to the nontreated diabetic group. Among the UPR markers, those involved in the protein kinase-like ER kinase (PERK) pathway were increased, while UDCA attenuated UPR in STZ- induced diabetic mice as well as AGE- or mLDL-exposed retinal pericytes in culture. Consequently, vascular integrity was improved and pericyte loss reduced in the retina of STZ-induced diabetic mice. Our findings suggest that UDCA might be effective in protecting against DR.
provenance
Made available in Cube on 2018-09-28T16:16:30Z (GMT). No. of bitstreams: 0
language
English
author
Chung, Yoo-Ri
Choi, Jeong A.
Koh, Jae-Young
Yoon, Young Hee
accessioned
2018-09-28T16:16:30Z
available
2018-09-28T16:16:30Z
issued
2017
citation
JOURNAL OF DIABETES RESEARCH
issn
2314-6745
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486771.do
Funder
미래창조과학부
Funding Program
중견연구자지원
Project ID
1711030063
Jurisdiction
Rep.of Korea
Project Name
Investigating the mechanisms of photoreceptor degeneration and cell death and discovering a treatment modality
rights
openAccess
type
article


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