Unique Features of Aeromonas Plasmid pAC3 and Expression of thePlasmid-Mediated Quinolone Resistance Genes

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486725.do
DOI
10.1128/mSphere.00203-17
Title
Unique Features of Aeromonas Plasmid pAC3 and Expression of thePlasmid-Mediated Quinolone Resistance Genes
Description
C.N.T. is grateful for a CAYSS scholarship to Chung-Ang University. Thiswork was supported by the National Research Foundation of Korea(NRF-2013R1A1A2012270), Ministry of Education, Science & Technology, andfunded by the Korean Ministry of Environment (MOE) as the EnvironmentalHealth Action Program (2016001350004). The authors declare no conflictof interest.
abstract
A highly fluoroquinolone-resistant isolate of Aeromonas species was isolated from a wastewater treatment plant and found to possess multiple resistance mechanisms, including mutations in gyrA and parC, efflux pumps, and plasmid-mediated quinolone resistance (PMQR) genes. Complete sequencing of the IncU-type plasmid, pAC3, present in the strain revealed a circular plasmid DNA 15,872 bp long containing two PMQR genes [qnrS2 and aac(6')-Ib-cr]. A mobile insertion cassette element containing the qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure was identified in the plasmid. The present study revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Plasmid pAC3 was introduced into Escherichia coli, and its PMQR genes were expressed, resulting in the acquisition of resistance. Proteome analysis of the recipient E. coli strain harboring the plasmid revealed that aac(6')-Ib-cr expression was constitutive and that qnrS2 expression was dependent upon fluoroquinolone stress through regulation by regulator of sigma D (Rsd). To the best of our knowledge, this is the first report to characterize a novel MITE sequence upstream of the PMQR gene within a mobile insertion cassette, as well as the regulation of qnrS2 expression. Our results suggest that this mobile element may play an important role in qnrS2 dissemination.
IMPORTANCE In the present study, plasmid pAC3 isolated from a highly fluoroquinolone-resistant isolate of Aeromonas species was sequenced and found to contain two fluoroquinolone resistance genes, aac(6')-Ib-cr and qnrS2. Comparative analyses of plasmid pAC3 and other Aeromonas sp. IncU-type plasmids revealed a mobile insertion cassette element with a unique structure containing a qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure. This study also revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Our results a
provenance
Made available in Cube on 2018-09-28T16:15:16Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Dae-Wi
Thawng, Cung Nawl
Lee, Sang Hee
Cha, Chang-Jun
orcid
Lee, Sang Hee/0000-0002-5660-7122
accessioned
2018-09-28T16:15:16Z
available
2018-09-28T16:15:16Z
issued
2017
citation
MSPHERE(2): 3
issn
2379-5042
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486725.do
Funder
환경부
Funding Program
생활공감환경보건기술
Project ID
1485014454
Jurisdiction
Rep.of Korea
Project Name
Understanding of emergence of antibiotic resistance in the environment and development of technologies for construction of national monitoring system
rights
openAccess
subject
Aeromonas
plasmid-mediated quinolone resistance
aac(6 ')-Ib-cr
miniature inverted-repeat transposable element
qnrS2
regulator ofsigma D
type
article


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