Umbilical cord-derived mesenchymal stem cell extracts reduce colitis inmice by re-polarizing intestinal macrophages

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486694.do
DOI
10.1038/s41598-017-09827-5
Title
Umbilical cord-derived mesenchymal stem cell extracts reduce colitis inmice by re-polarizing intestinal macrophages
Description
This work was supported by a grant from the Asan Institute for LifeSciences (grant number 2014-309) and a grant from the Korea HealthTechnology R&D Project through the Korea Health Industry DevelopmentInstitute (KHIDI), Ministry of Health, Welfare and Family Affairs,Republic of Korea (HI15C2111).
abstract
Human umbilical cord mesenchymal stem cells (hUC-MSCs), originating in Wharton's jelly, are multipotent stem cells that home to damaged tissues and can modulate the immune system. We examined whether administering extracts of MSCs (MSC-Ex) instead of MSCs could augment the beneficial effects of MSC therapy by overcoming the low homing efficiency of MSCs systemically administered in inflammatory bowel diseases (IBD). Dextran sodium sulfate-induced colitis model was established in C57BL/6 mice, and MSC-Ex was administered intraperitoneally. MSC-Ex reduced colitis, disease activity index (DAI), and histological colitis scores, and increased the body weight. Treatment with MSC-Ex completely blocked the induction of inflammatory cytokines, which were strongly detected in mice with colitis. MSC-Ex shifted the macrophage functional phenotype from M1 to M2 by decreasing the levels of MCP1, CXCL9, and iNOS, but increasing the levels of IL-10, LIGHT, CCL1, and Arg-1. MSC-Ex recovered the destruction of the epithelial barrier in the differentiated Caco-2 cells in vitro. Treatment with MSC-Ex was more potent than that with MSC in reducing DAI, the histological score, and nitrite levels. These data strongly support that MSC-Ex treatment can be a potent approach to overcome severe refractory IBD.
provenance
Made available in Cube on 2018-09-28T16:14:27Z (GMT). No. of bitstreams: 0
language
English
author
Song, Ji-young
Kang, Hyo Jeong
Hong, Joon Seok
Kim, Chong Jai
Shim, Jae-Yoon
Lee, Christopher W.
Choi, Jene
accessioned
2018-09-28T16:14:27Z
available
2018-09-28T16:14:27Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486694.do
Funder
보건복지부
Funding Program
질환극복기술개발
Project ID
1465024499
Jurisdiction
Rep.of Korea
Project Name
Mesenchymal stem cell extract injection therapy for inflammatory bowel disease
rights
openAccess
type
article


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