Ultra-sensitive detection of kanamycin for food safety using a reducedgraphene oxide-based fluorescent aptasensor

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486672.do
DOI
10.1038/srep40305
Title
Ultra-sensitive detection of kanamycin for food safety using a reducedgraphene oxide-based fluorescent aptasensor
Description
This work was supported by the Agricultural Biotechnology DevelopmentProgram, Ministry of Agriculture, Food, and Rural Affairs, Republic ofKorea (314013-3).
abstract
Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar K-d(app) values with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety.
provenance
Made available in Cube on 2018-09-28T16:13:51Z (GMT). No. of bitstreams: 0
language
English
author
Ha, Na-Reum
Jung, In-Pil
La, Im-Joung
Jung, Ho-Sup
Yoon, Moon-Young
accessioned
2018-09-28T16:13:51Z
available
2018-09-28T16:13:51Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486672.do
Funder
농림축산식품부
Funding Program
농생명산업기술개발
Project ID
1545012811
Jurisdiction
Rep.of Korea
Project Name
색변이 앱타센서를 이용한 도축 전 고감도 항생물질 검사 키트 개발
rights
openAccess
type
article


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