Type-dependent action modes of TtAA9E and TaAA9A acting on cellulose anddifferently pretreated lignocellulosic substrates

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486618.do
DOI
10.1186/s13068-017-0721-4
Title
Type-dependent action modes of TtAA9E and TaAA9A acting on cellulose anddifferently pretreated lignocellulosic substrates
Description
We want to acknowledge grant support from C1 Gas Refinery Programthrough the National Research Foundation of Korea funded by MSIP(2016M3D3A1A01913143).
abstract
Background: Lytic polysaccharide monooxygenase (LPMO) is a group of recently identified proteins that catalyze oxidative cleavage of the glycosidic linkages of cellulose and other polysaccharides. By utilizing the oxidative mode of action, LPMOs are able to enhance the efficiency of cellulase in the hydrolysis of cellulose. Particularly, auxiliary activity family 9 (AA9) is a group of fungal LPMOs that show a type-dependent regioselectivity on cellulose in which Types 1, 2, and 3 hydroxylate at C1, C4, and C1 and C4 positions, respectively. In this study, we investigated comparative characteristics of TtAA9E from Thielavia terrestris belonging to Type 1 and TaAA9A from Thermoascus aurantiacus belonging to Type 3 on cellulose and pretreated lignocellulose.
Results: From product analysis, TtAA9E dominantly generated oligosaccharides with an aldonic acid form, which is an evidence of C1 oxidation, while TaAA9A generated oligosaccharides with both aldonic acid and 4-ketoaldose forms, which is evidence of C1 and C4 oxidations, respectively. For hydrolysis of cellulose (Avicel) by cellulase, higher synergism was observed for TtAA9E than for TaAA9A. For hydrolysis of pretreated lignocellulose using rice straw, synergistic behaviors of TtAA9E and TaAA9A were different depending on the pretreatment of rice straw. Specifically, on acid-pretreated rice straw, TtAA9E showed a higher synergism than TaAA9A while on alkali-pretreated rice straw, TaAA9A showed a higher synergism than TtAA9E.
Conclusions: We show type-dependent action modes of TtAA9E and TaAA9A for cellulose oxidation together with substrate-dependent synergistic hydrolysis of cellulosic substrates. The results obtained from this study indicate the different behaviors of AA9s on cellulose and pretreated lignocellulose, suggesting a selection of AA9 proteins specific to substrates is required for industrial utilization.
provenance
Made available in Cube on 2018-09-28T16:12:25Z (GMT). No. of bitstreams: 0
language
English
author
Kim, In Jung
Seo, Nari
An, Hyun Joo
Kim, Jae-Han
Harris, Paul V.
Kim, Kyoung Heon
orcid
Kim, Kyoung Heon/0000-0003-4600-8668
accessioned
2018-09-28T16:12:25Z
available
2018-09-28T16:12:25Z
issued
2017
citation
BIOTECHNOLOGY FOR BIOFUELS(10)
issn
1754-6834
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486618.do
Funder
과학기술정보통신부
Funding Program
기후변화대응기술개발
Project ID
1711048841
Jurisdiction
Rep.of Korea
Project Name
Development of biological technology for the production of n-C6 acid from waste gas containing CO
rights
openAccess
subject
Auxiliary activity family 9
Lytic polysaccharide monooxygenase
Glycoside hydrolase family 61
Cellulose oxidation
Synergism
Cellulosehydrolysis
type
article


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