Two NADPH: Protochlorophyllide Oxidoreductase (POR) Isoforms PlayDistinct Roles in Environmental Adaptation in Rice

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486593.do
DOI
10.1186/s12284-016-0141-2
Title
Two NADPH: Protochlorophyllide Oxidoreductase (POR) Isoforms PlayDistinct Roles in Environmental Adaptation in Rice
Description
We thank Yong-Jae Kim at the National Center for Inter-UniversityResearch Facilities (NCIRF) for technical assistance with CLSM analysisand Jihyeon Choi for technical support with the Cary Eclipsefluorescence spectrophotometer. This work was carried out with thesupport of the Cooperative Research Program for Agriculture & TechnologyDevelopment (Project No. PJ011063), Rural Development Administration,Republic of Korea. Choon-Tak Kwon was supported by a postdoctoralfellowship from the Basic Science Research Program(NRF-2015R1A6A3A01057535) through the National Research Foundation ofKorea (NRF) funded by the Ministry of Education.
abstract
NADPH: protochlorophyllide oxidoreductase (POR) is an essential enzyme that catalyzes the photoreduction of protochlorophyllide to chlorophyllide, which is ultimately converted to chlorophyll in developing leaves. Rice has two POR isoforms, OsPORA and OsPORB. OsPORA is expressed in the dark during early leaf development; OsPORB is expressed throughout leaf development regardless of light conditions. The faded green leaf (fgl) is a loss-of-function osporB mutant that displays necrotic lesions and variegation in the leaves due to destabilized grana thylakoids, and has increased numbers of plastoglobules in the chloroplasts. To investigate whether the function of OsPORA can complement that of OsPORB, we constitutively overexpressed OsPORA in fgl mutant.
In the 35S:OsPORA/fgl (termed OPAO) transgenic plants, the necrotic lesions of the mutant disappeared and the levels of photosynthetic pigments and proteins, as well as plastid structure, were recovered in developing leaves under natural long days in the paddy field and under short days in an artificially controlled growth room. Under constant light conditions, however, total chlorophyll and carotenoid levels in the developing leaves of OPAO plants were lower than those of wild type. Moreover, the OPAO plants exhibited mild defects in mature leaves beginning at the early reproductive stage in the paddy field.
The physiological function of OsPORB in response to constant light or during reproductive growth cannot be completely replaced by constitutive activity of OsPORA, although the biochemical functions of OsPORA and OsPORB are redundant. Therefore, we suggest that the two OsPORs have differentiated over the course of evolution, playing distinct roles in the adaptation of rice to the environment.
provenance
Made available in Cube on 2018-09-28T16:11:46Z (GMT). No. of bitstreams: 0
language
English
author
Kwon, Choon-Tak
Kim, Suk-Hwan
Song, Giha
Kim, Dami
Paek, Nam-Chon
accessioned
2018-09-28T16:11:46Z
available
2018-09-28T16:11:46Z
issued
2017
citation
RICE(10)
issn
1939-8425
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486593.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274242
Jurisdiction
Rep.of Korea
Project Name
Research team for crop genomics and breeding
rights
openAccess
subject
Rice
Faded green leaf
OsPORA
OsPORB
Chlorophyll synthesis
type
article


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