Twin birth changes DNA methylation of subsequent siblings

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486583.do
DOI
10.1038/s41598-017-08595-6
Title
Twin birth changes DNA methylation of subsequent siblings
Description
We would like to thank the subjects participating in each study. Thedata analysis was facilitated by Spartan, the High Performance Computingsystem (HPC) of the University of Melbourne. The AMDTSS was facilitatedthrough the Australian Twin Registry, a national research resource inpart supported by a Centre for Research Excellence Grant from theNational Health and Medical Research Council (NHMRC) APP 1079102. TheAMDTSS was supported by NHMRC (grant numbers 1050561 and 1079102),Cancer Australia and National Breast Cancer Foundation (grant number509307). The KHTS was supported by a fund (2014-E71004-00) by Researchof Korea Centers for Disease Control, and Prevention and Basic ScienceResearch Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Science, ICT & Future Planning (grant numberNRF-2017R1A2B2002136). SL is supported by the Australian GovernmentResearch Training Program Scholarship and Richard Lovell TravellingScholarship from the University of Melbourne. TLN is supported by aNHMRC Post-Graduate Scholarship and the Richard Lovell TravellingScholarship from the University of Melbourne. MCS is a Senior ResearchFellow of NHMRC. JLH is a Senior Principal Research Fellow of NHMRC anda Distinguished Visiting Professor at Seoul National University.
abstract
We asked if twin birth influences the DNA methylation of subsequent siblings. We measured whole blood methylation using the HumanMethylation450 array for siblings from two twin and family studies in Australia and Korea. We compared the means and correlations in methylation between pairs of siblings born before a twin birth (BT siblings), born on either side of a twin birth (B/AT pairs) and born after a twin birth (AT siblings). For the genome-wide average DNA methylation, the correlation for AT pairs (r(AT)) was larger than the correlation for BT pairs (r(BT)) in both studies, and from the meta-analysis, r(AT) = 0.46 (95% CI: 0.26, 0.63) and r(BT) = -0.003 (95% CI: -0.30, 0.29) (P = 0.02). B/AT pairs were not correlated (from the meta-analysis r(BAT) = 0.08; 95% CI: -0.31, 0.45). Similar results were found for the average methylation of several genomic regions, e.g., CpG shelf and gene body. BT and AT pairs were differentially correlated in methylation for 15 probes (all P < 10(-7)), and the top 152 differentially correlated probes (at P < 10(-4)) were enriched in cell signalling and breast cancer regulation pathways. Our observations are consistent with a twin birth changing the intrauterine environment such that siblings both born after a twin birth are correlated in DNA methylation.
provenance
Made available in Cube on 2018-09-28T16:11:31Z (GMT). No. of bitstreams: 0
language
English
author
Li, Shuai
Kim, Eunae
Wong, Ee Ming
Joo, Ji-Hoon Eric
Nguyen, Tuong L.
Stone, Jennifer
Song, Yun-Mi
Flander, Louisa B.
Saffery, Richard
Giles, Graham G.
Southey, Melissa C.
Sung, Joohon
Hopper, John L.
orcid
Li, Shuai/0000-0002-8696-8594; Stone, Jennifer/0000-0001-5077-0124;
Nguyen, Tuong/0000-0002-6597-8363; Saffery, Richard/0000-0002-9510-4181
accessioned
2018-09-28T16:11:31Z
available
2018-09-28T16:11:31Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486583.do
Funder
과학기술정보통신부
Funding Program
개인기초연구(미래부)
Project ID
1711050704
Jurisdiction
Rep.of Korea
Project Name
Identifying DNA Methylation Signature for Environmental Exposure: Developing Novel Exposure Markers of Humidifier Disinfectants
rights
openAccess
type
article


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