Turning behaviors of T cells climbing up ramp-like structures areregulated by myosin light chain kinase activity and lamellipodiaformation

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486578.do
DOI
10.1038/s41598-017-11938-y
Title
Turning behaviors of T cells climbing up ramp-like structures areregulated by myosin light chain kinase activity and lamellipodiaformation
Description
This work was supported by Institute for Information & communicationsTechnology Promotion (IITP) grant (No. 20170009530012003), a grant ofthe Korea Health Technology R&D Project through the Korea HealthIndustry Development Institute (KHIDI) funded by the Ministry of Health& Welfare of Korea (grant number: HI15C0001), and National ResearchFoundation of Korea (NRF) grant (No. NRF-2017R1A2B3006006) funded by theKorea government (MSIP).
abstract
T cells navigate diverse microenvironments to perform immune responses. Micro-scale topographical structures within the tissues, which may inherently exist in normal tissues or may be formed by inflammation or injury, can influence T cell migration, but how T cell migration is affected by such topographical structures have not been investigated. In this study, we fabricated ramp-like structures with a 5 mu m height and various slopes, and observed T cells climbing up the ramp-like structures. T cells encountering the ramp-like structures exhibited MLC accumulation near head-tail junctions contacting the ramp-like structures, and made turns to the direction perpendicular to the ramp-like structures. Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures. Arp2/3 or MLCK inhibition substantially reduced probability of T cells climbing sharp-edged ramp-like structures, indicating intriguing turning behavior of T cells mediated by lamellipodia formation and MLCK activity may be important for T cells to access inflamed or injured tissues with abrupt topographical changes.
provenance
Made available in Cube on 2018-09-28T16:11:23Z (GMT). No. of bitstreams: 0
language
English
author
Song, Kwang Hoon
Lee, Jaehyun
Jung, Hong-Ryul
Park, HyoungJun
Doh, Junsang
accessioned
2018-09-28T16:11:23Z
available
2018-09-28T16:11:23Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486578.do
Funder
산업통상자원부
Funding Program
로봇산업핵심기술개발
Project ID
1415152144
Jurisdiction
Rep.of Korea
Project Name
Development of robotic core technologies for patient-specific precision examination and keratoplasty based on fabrication of 50 μm-level layered artificial cornea
rights
openAccess
type
article


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