Tropism and infectivity of duck-derived egg drop syndrome virus inchickens

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486528.do
DOI
10.1371/journal.pone.0177236
Title
Tropism and infectivity of duck-derived egg drop syndrome virus inchickens
Description
This work was supported by Korea Institute of Planning and Evaluationfor Technology in Food, Agriculture, Forestry and Fisheries (314023-3,316039-3, 314004-3, 716002-7) and Research of Animal and PlantQuarantine Agency, South Korea. The funders had no role in study design,data collection and analysis, decision to publish, or preparation of themanuscript.
abstract
Egg drop syndrome virus (EDSV) can markedly decrease egg production in laying hens. Duck is the natural host of EDSV. EDSV derived from ducks abrogate egg drop in laying hens. We have previously confirmed that duck-derived EDSVs have a variety of replication activities in chick embryo liver (CEL) cells. However, it is currently unclear whether duck-derived EDSV could display tropism and adaptation in laying hens. This study assessed whether duck-derived EDSV can adapt to laying hens, and estimated the inducing factors. Complete genome sequences of duck-derived EDSVs (D11-JW-012, D11-JW-017, and D11-JW-032 isolates) with various replication efficiency in CEL cells and C10-GY-001 isolate causing disease in laying hens were analyzed to find their differences. Phylogenetic analysis of complete genome sequence revealed that C10-GY-001, D11-JW-032, and strain 127 virus as vaccine were clustered into the same group, with D11-JW-012 and D11-JW-017 clustered in another group. Comparison between D11-JW-012 isolate that poorly replicated and D11-JW-017 isolate that replicated well in CEL cells in same cluster revealed six amino acid differences on IVa2, DNA polymerase, endopeptidase, and DNA-binding protein. These amino acids might be key candidates enhancing cellular tropism in chicken. When the pathogenicities of these isolates in laying hens were compared, D11-JW-032 showed severe signs similar to 127 virus, D11-JW-017 showed intermediate signs, while D11-JW-012 showed almost no sign. Eleven amino acids differed between D11-JW-032 and D11-JW-017, and 17 amino acids were different between D11-JW-032 and D11-JW-012. These results suggest that EDSVs derived from ducks have various pathogenicities in laying hens. Key amino acid candidates might have altered their affinity to tropism of laying hens, causing difference pathogenicities.
provenance
Made available in Cube on 2018-09-28T16:10:02Z (GMT). No. of bitstreams: 0
language
English
author
Kang, Min
Cha, Se-Yeoun
Jang, Hyung-Kwan
accessioned
2018-09-28T16:10:02Z
available
2018-09-28T16:10:02Z
issued
2017
citation
PLOS ONE(12): 5
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486528.do
Funder
농림축산식품부
Funding Program
가축질병대응기술개발
Project ID
1545014145
Jurisdiction
Rep.of Korea
Project Name
육용오리 AI 예찰 및 경보시스템 구축 기반기술 학립
rights
openAccess
type
article


Files in This Item

There are no attached files.