Transthyretin: A Transporter Protein Essential for Proliferation ofMyoblast in the Myogenic Program

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486490.do
DOI
10.3390/ijms18010115
Title
Transthyretin: A Transporter Protein Essential for Proliferation ofMyoblast in the Myogenic Program
Description
All research materials used in this study were provided by the BovineGenome Resources Bank of Yeungnam University (Gyeongsan, Korea). Thisresearch was supported by the Basic Science Research Program through theNational Research Foundation of Korea (NRF) funded by the Ministry ofEducation (2016R1C1B1011478) and a National Research Foundation of Korea(NRF) grant funded by the Korean government (MSIP; Grant No.2014R1A2A2A01006324).
abstract
Irregularities in the cellular uptake of thyroid hormones significantly affect muscle development and regeneration. Herein, we report indispensable role of transthyretin (TTR) in maintaining cellular thyroxine level. TTR was found to enhance recruitment of muscle satellite cells to the site of injury, thereby regulating muscle regeneration. Fluorescence-activated cell sorting (FACS) and immunofluorescence analysis of TTRwt (TTR wild type) and TTRkd (TTR knock-down) cells revealed that TTR controlled cell cycle progression by affecting the expression of Cyclin A2. Deiodinase 2 (D2) mediated increases in triiodothyronine levels were found to regulate the expression of myogenic marker, myogenin (MYOG). Moreover, use of a coumarin derivative (CD) revealed a significant reduction in cellular thyroxine, thereby indicating that TTR play a role in the transport of thyroxine. Taken together, these findings suggest that TTR mediated transport of thyroxine represents a survival mechanism necessary for the myogenic program. The results of this study will be highly useful to the strategic development of novel therapeutics to combat muscular dystrophies.
provenance
Made available in Cube on 2018-09-28T16:09:00Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Eun Ju
Pokharel, Smritee
Jan, Arif Tasleem
Huh, Soyeon
Galope, Richelle
Lim, Jeong Ho
Lee, Dong-Mok
Choi, Sung Wook
Nahm, Sang-Soep
Kim, Yong-Woon
Park, So-Young
Choi, Inho
accessioned
2018-09-28T16:09:00Z
available
2018-09-28T16:09:00Z
issued
2017
citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(18): 1
issn
1422-0067
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486490.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274458
Jurisdiction
Rep.of Korea
Project Name
Creative Human Resource Project for Demand Directed New Drug Development
rights
openAccess
subject
myoblast
transthyretin
proliferation
differentiation
type
article


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