Transition of colistin dependence into colistin resistance inAcinetobacter baumannii

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486459.do
DOI
10.1038/s41598-017-14609-0
Title
Transition of colistin dependence into colistin resistance inAcinetobacter baumannii
Description
This research was supported by the Basic Science Research Programthrough the National Research Foundation of Korea (NRF), funded by theMinistry of Science, ICT and Future Planning (Grant NumberNRF-2016R1A2A2A05005075). JY Lee was partly supported by the BasicScience Research Program through the NRF funded by the Ministry ofEducation (grant no. NRF-2016R1A6A3A11931488).
abstract
We recently demonstrated a high rate of colistin dependence in Acinetobacter baumannii isolates exposed to colistin in vitro. In the present study, we obtained a colistin-resistant (H08-391R) and colistin-dependent mutant (H08-391D) from a colistin-susceptible parental strain (H08-391). We found that the colistin-dependent mutant converted into a stable colistin-resistant mutant (H08-391D-R) in vitro after four serial passages without colistin. H08-391D and H08-391D-R were both found to harbor defective lipid A, as indicated by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis. Additionally, both contained an ISAba1 insertion in lpxC, which encodes a lipid A biosynthetic enzyme. Further, membrane potential measurements using the fluorescent dye 3,3'-diethyloxacarbocyanine iodide (DiOC(2)[3]) showed that the membrane potential of H08-391D and H08-391D-R was significantly decreased as compared to that of the parental strain, H08-391. Moreover, these mutant strains exhibited increased susceptibilities to antibiotics other than colistin, which may be attributed to their outer membrane fragility. Such phenomena were identified in other A. baumannii strains (H06-855 and its derivatives). Taken together, our study reveals that the colistin-dependent phenotype is a transient phenotype that allows A. baumannii to survive under colistin pressure, and can transition to the extremely resistant phenotype, even in an antibiotic-free environment.
provenance
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language
English
author
Lee, Ji-Young
Chung, Eun Seon
Ko, Kwan Soo
accessioned
2018-09-28T16:08:12Z
available
2018-09-28T16:08:12Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486459.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274065
Jurisdiction
Rep.of Korea
Project Name
21st Century Biomedical Sciences Leader Development Program
rights
openAccess
type
article


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