Transient Depletion of CD169(+) Cells Contributes to Impaired EarlyProtection and Effector CD8(+) T Cell Recruitment against MucosalRespiratory Syncytial Virus Infection

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486449.do
DOI
10.3389/fimmu.2017.00819
Title
Transient Depletion of CD169(+) Cells Contributes to Impaired EarlyProtection and Effector CD8(+) T Cell Recruitment against MucosalRespiratory Syncytial Virus Infection
Description
This study was supported by the National Research Foundation(NRF-2016R1A2B2015028, NRF-2015R1A4A1042416, and NRF-2013R1A1A2063347)and KAIST Future Systems Healthcare Project funded by the Ministry ofScience, ICT, and Future Planning of Korea.
abstract
Respiratory syncytial virus (RSV) is a major cause of respiratory viral infections in infants and children. Alveolar macrophages (AMs) play a crucial role in combatting airborne pathogens, strongly express CD169, and are localized in the lung alveoli. Therefore, we used CD169-diphtheria toxin receptor (DTR) transgenic mice to explore the roles of CD169(+) cells in immune responses to mucosal RSV infection. The administration of diphtheria toxin to CD169-DTR mice induced specific AM depletion and reduced the recruitment of Ly6C(hi) monocytes. Notably, CD169(+) cell depletion reduced levels of innate cytokines, such as interferon-beta, IL-6, and TNF-alpha, in bronchoalveolar lavage fluid during RSV infection without affecting the production of proinflammatory chemokines. Moreover, the depletion of CD169(+) cells increased the recruitment of inflammatory cells to the lung during the early stage of RSV infection, although not during the later stages of RSV infection. Furthermore, the depletion of CD169(+) cells reduced the recruitment of effector CD8(+) T cells to the lungs after RSV mucosal infection. Our findings suggest that modulating the number of CD169(+) cells to enhance immune responses to RSV infection may be useful as a new therapeutic strategy.
provenance
Made available in Cube on 2018-09-28T16:07:56Z (GMT). No. of bitstreams: 0
language
English
author
Oh, Dong Sun
Oh, Ji Eun
Jung, Hi Eun
Lee, Heung Kyu
orcid
Lee, Heung Kyu/0000-0002-3977-1510
accessioned
2018-09-28T16:07:56Z
available
2018-09-28T16:07:56Z
issued
2017
citation
FRONTIERS IN IMMUNOLOGY(8)
issn
1664-3224
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486449.do
Funder
과학기술정보통신부
Funding Program
한국과학기술원연구운영비지원(0.5)
Project ID
1711063537
Jurisdiction
Rep.of Korea
Project Name
Systems Healthcare
rights
openAccess
subject
alveolar macrophage
respiratory syncytial virus
type I interferon
CD169
CXCL9/10
type
article


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