Transcriptomic Analysis of Insulin-Sensitive Tissues from Anti-DiabeticDrug Treated ZDF Rats, a T2DM Animal Model

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486425.do
DOI
10.1371/journal.pone.0069624
Title
Transcriptomic Analysis of Insulin-Sensitive Tissues from Anti-DiabeticDrug Treated ZDF Rats, a T2DM Animal Model
Description
This work was supported by the grant from the Korea Food and DrugAdministration (1470001450) to SJK and KKS; a grant by National ResearchFoundation (NRF), which is funded by the Korean government(MEST)(2012055344 and 2012M3A9D1054622) to SJK. This study was alsopartially supported by the Research Institute for Veterinary Science,Seoul National University. SJK, KYN, SJH, and KIY were supported by theBrain Korea 21 Program for Veterinary Science. The funders had no rolein study design, data collection and analysis, decision to publish, orpreparation of the manuscript.
abstract
Gene expression changes have been associated with type 2 diabetes mellitus (T2DM); however, the alterations are not fully understood. We investigated the effects of anti-diabetic drugs on gene expression in Zucker diabetic fatty (ZDF) rats using oligonucleotide microarray technology to identify gene expression changes occurring in T2DM. Global gene expression in the pancreas, adipose tissue, skeletal muscle, and liver was profiled from Zucker lean control (ZLC) and anti-diabetic drug treated ZDF rats compared with those in ZDF rats. We showed that anti-diabetic drugs regulate the expression of a large number of genes. We provided a more integrated view of the diabetic changes by examining the gene expression networks. The resulting sub-networks allowed us to identify several biological processes that were significantly enriched by the antidiabetic drug treatment, including oxidative phosphorylation (OXPHOS), systemic lupus erythematous, and the chemokine signaling pathway. Among them, we found that white adipose tissue from ZDF rats showed decreased expression of a set of OXPHOS genes that were normalized by rosiglitazone treatment accompanied by rescued blood glucose levels. In conclusion, we suggest that alterations in OXPHOS gene expression in white adipose tissue may play a role in the pathogenesis and drug mediated recovery of T2DM through a comprehensive gene expression network study after multidrug treatment of ZDF rats.
provenance
Made available in Cube on 2018-09-28T16:07:17Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Yo Na
Kim, Sangok
Kim, Il-Yong
Shin, Jae Hoon
Cho, Sooyoung
Yi, Sun Shin
Kim, Wan Kyu
Kim, Kyung-Sub
Lee, Sanghyuk
Seong, Je Kyung
accessioned
2018-09-28T16:07:17Z
available
2018-09-28T16:07:17Z
issued
2013
citation
PLOS ONE(8): 7
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486425.do
Funder
미래창조과학부
Funding Program
바이오·의료기술개발
Project ID
1345202188
Jurisdiction
Rep.of Korea
Project Name
Integrative analysis of genomics data for the discovery of proteogenomic molecular signature
rights
openAccess
type
article


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