Transcriptome analysis of the oriental melon (Cucumis melo L. var.makuwa) during fruit development

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486407.do
DOI
10.7717/peerj.2834
Title
Transcriptome analysis of the oriental melon (Cucumis melo L. var.makuwa) during fruit development
Description
This work was supported by a grant from the Biogreen21 program(PJ011088) of the Rural Development Administration, Korea, and the KRIBBResearch Initiative Program. The funders had no role in study design,data collection and analysis, decision to publish, or preparation of themanuscript.
abstract
Background. The oriental melon (Cucumis melo L. var. makuwa) is one of the most important cultivated cucurbits grown widely in Korea, Japan, and northern China It is cultivated because its fruit as a sweet aromatic flavor and is rich soluble sugars,orgnic acids, minerals, and vitamins. In order to elucidate the genetic and molecular basis of the developmental changes that determine size, color, and sugar contents of the fruit, we performed de novo transcriptorne sequencing to analyze the genes expressed during fruit development.
Results. We identified a total of 147,6661 of representative loci from 100,875 transcnpts and functionally annotated 33,963 of the loci based on orthologs in Arabidopsis thaliana. Among those loci, we identified 5,173 differentially expressed genes, which were classified into 14 clusters base on the modulation of their expression patterns. The expression patterns suggested that the differentially expressed genes were related to fruit development and maturation through diverse metabolic pathways. Analyses based on gene set enrichment and the pathways described in the Kyoto Encyclopedia of Genes and Genomes suggested that the expression of genes involved in starch and sucrose metabolism and Icarotenoid biosynthesis were regulated dynamically during fruit development and subsequent maturation.
Conclusion. Our results provide the gene expression patterns related to different stages of fruit development and maturation in the oriental melon. The expression patterns give clues about important regulatory mechanisms, especially those involving starch, sugar, and carotenoid biosynthesis, in the development of the oriental melon fruit.
provenance
Made available in Cube on 2018-09-28T16:06:47Z (GMT). No. of bitstreams: 0
language
English
author
Shin, Ah-Young
Kim, Yong-Min
Koo, Namjin
Lee, Su Min
Nahm, Seokhyeon
Kwon, Suk-Yoon
accessioned
2018-09-28T16:06:47Z
available
2018-09-28T16:06:47Z
issued
2017
citation
PEERJ(5)
issn
2167-8359
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486407.do
Funder
농촌진흥청
Funding Program
차세대바이오그린21
Project ID
1395045019
Jurisdiction
Rep.of Korea
Project Name
Multiple references of Cucubits (melon) for GWAS
rights
openAccess
subject
Korean melon
Transcriptome
Fruit development
Pathway analysis
Sucrose
Carotenoids
type
article


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