Transcriptional Regulation of Cellulose Biosynthesis during the EarlyPhase of Nitrogen Deprivation in Nannochloropsis salina

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486401.do
DOI
10.1038/s41598-017-05684-4
Title
Transcriptional Regulation of Cellulose Biosynthesis during the EarlyPhase of Nitrogen Deprivation in Nannochloropsis salina
Description
This work was supported by grants from the Advanced Biomass R&D Center(ABC) of Korea (NRF-2015M3A6A2065747, NRF-2011-0031350 andNRF-2010-0029728), the Ministry of Science, ICT and Future Planning,Korea.
abstract
Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N-), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the celluloserich cell wall should change in response to N- stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis. However, few studies have characterised microalgal cell wall metabolism, including oleaginous Nannochloropsis sp. microalgae subjected to nitrogen deficiency. Here, we investigated N-induced changes in cellulose biosynthesis in N. salina. We observed that N-induced cell wall thickening, concurrently increased the transcript levels of genes coding for UDPG pyrophosphorylase and cellulose synthases, and increased cellulose content. Nannochloropsis salina cells with thickened cell wall were more susceptible to mechanical stress such as bead-beating and sonication, implicating cellulose metabolism as a potential target for cost-effective microalgal cell disruption.
provenance
Made available in Cube on 2018-09-28T16:06:38Z (GMT). No. of bitstreams: 0
language
English
author
Jeong, Seok Won
Nam, Seung Won
HwangBo, Kwon
Jeong, Won Joong
Jeong, Byeong-ryool
Chang, Yong Keun
Park, Youn-Il
orcid
Jeong, Byeong-ryool/0000-0003-0642-1650
accessioned
2018-09-28T16:06:38Z
available
2018-09-28T16:06:38Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486401.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274024
Jurisdiction
Rep.of Korea
Project Name
Program for Multi-scale Chemical & Biomolecular Engineering
rights
openAccess
type
article


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