Toxic effects of atrazine on porcine oocytes and possible mechanisms ofaction

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486373.do
DOI
10.1371/journal.pone.0179861
Title
Toxic effects of atrazine on porcine oocytes and possible mechanisms ofaction
Description
This study was supported by the BioGreen 21 Program (No. PJ011126) andthe National Natural Science Foundation of China (31572400, 31501954).The funders had no role in study design, data collection and analysis,decision to publish, or preparation of the manuscript.
abstract
Because atrazine is a widely used herbicide, its adverse effects on the reproductive system have been extensively researched. In this study, we investigated the effects of atrazine exposure on porcine oocyte maturation and the possible mechanisms. Our results showed that the rates of oocyte maturation significantly decreased after treatment with 200 mu M atrazine in vitro. Atrazine treatment resulted in abnormal spindle morphology but did not affect actin distribution. Atrazine exposure not only triggered a DNA damage response but also decreased MPF levels in porcine oocytes. Our results also revealed that atrazine worsened porcine oocyte quality by causing excessive accumulation of superoxide radicals, increasing cathepsin B activity, and decreasing the GSH level and mitochondrial membrane potential. Furthermore, atrazine decreased developmental competence of porcine oocytes up to the blastocyst stage and changed some properties: cell numbers, apoptosis, and related gene expression levels. Collectively, our results indicate that porcine oocyte maturation is defective after atrazine treatment at least through disruption of spindle morphology, MPF activity, and mitochondrial function and via induction of DNA damage, which probably reduces developmental competence.
provenance
Made available in Cube on 2018-09-28T16:05:52Z (GMT). No. of bitstreams: 0
language
English
author
Yuan, Bao
Liang, Shuang
Jin, Yong-Xun
Zhang, Ming-Jun
Zhang, Jia-Bao
Kim, Nam-Hyung
accessioned
2018-09-28T16:05:52Z
available
2018-09-28T16:05:52Z
issued
2017
citation
PLOS ONE(12): 6
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486373.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274058
Jurisdiction
Rep.of Korea
Project Name
Brain Korea 21 Center for Bio-Resource Development
rights
openAccess
type
article


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