Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase andNF-kappa B Activation

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486347.do
DOI
10.1155/2017/7250968
Title
Torilin Inhibits Inflammation by Limiting TAK1-Mediated MAP Kinase andNF-kappa B Activation
Description
This research was supported by the National Research Foundation of KoreaGrant funded by the Korean government (Grant no. 2015R1D1A1A09057204)and by Research Base Construction Fund Support Program funded by ChonbukNational University in 2016.
abstract
Torilin, a sesquiterpene isolated from the fruits of Torilis japonica, has shown antimicrobial, anticancer, and anti-inflammatory properties. However, data on the mechanism of torilin action against inflammation is limited. This study aimed at determining the anti-inflammatory property of torilin in LPS-induced inflammation using in vitro model of inflammation. We examined torilin's effect on expression levels of inflammatory mediators and cytokines in LPS-stimulated RAW264.7 macrophages. The involvement of NF-kB and AP-1, MAP kinases, and adaptor proteins were assessed. Torilin strongly inhibited LPS-induced NO release, iNOS, PGE(2), COX-2, NF-alpha, IL-1 beta, IL-6, and GM-CSF gene and protein expressions. In addition, MAPKs were also suppressed by torilin pretreatment. Involvement of ERK1/2, P38(MAPK), and JNK1/2 was further confirmed by PD98059, SB203580, and SP600125 mediated suppression of iNOS and COX-2 proteins. Furthermore, torilin attenuated NF-kB and AP-1 translocation, DNA binding, and reporter gene transcription. Interestingly, torilin inhibited TAK1 kinase activation with the subsequent suppression of MAPK-mediated JNK, p38, ERK1/2, and AP-1 (ATF-2 and c-jun) activation and IKK-mediated I-kappa B alpha degradation, p65/p50 activation, and translocation. Together, the results revealed the suppression of NF-kappa B and AP-1 regulated inflammatory mediator and cytokine expressions, suggesting the test compound's potential as a candidate anti-inflammatory agent.
provenance
Made available in Cube on 2018-09-28T16:05:10Z (GMT). No. of bitstreams: 0
language
English
author
Endale, Mehari
Kim, Tae-Hwan
Kwak, Yi-Seong
Kim, Na-Mi
Kim, Seung-Hyung
Cho, Jae Youl
Yun, Bong-Sik
Rhee, Man-Hee
orcid
Endale, Mehari/0000-0002-1224-9415; Rhee, Man Hee/0000-0002-3088-1318
accessioned
2018-09-28T16:05:10Z
available
2018-09-28T16:05:10Z
issued
2017
citation
MEDIATORS OF INFLAMMATION
issn
0962-9351
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486347.do
Funder
교육부
Funding Program
개인기초연구(교육부)
Project ID
1345269961
Jurisdiction
Rep.of Korea
Project Name
The characterization of specific receptor for ginsenosides from Korean Red Ginseng
rights
openAccess
type
article


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