TonEBP/NFAT5 haploinsufficiency attenuates hippocampal inflammation inhigh-fat diet/streptozotocin-induced diabetic mice

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486325.do
DOI
10.1038/s41598-017-08319-w
Title
TonEBP/NFAT5 haploinsufficiency attenuates hippocampal inflammation inhigh-fat diet/streptozotocin-induced diabetic mice
Description
This research was supported by the Basic Science Research Programthrough the National Research Foundation (NRF) of Korea funded by theMinistry of Science, ICT and Future Planning (No. 2014R1A2A1A11049588and 2015R1A5A2008833).
abstract
Recent studies have shown that overexpression of tonicity-responsive enhancer binding protein (TonEBP) is associated with many inflammatory diseases, including diabetes mellitus, which causes neuroinflammation in the hippocampus as well as hepatic steatosis. However, the exact mechanism in diabetic neuroinflammation is unknown. We report that haploinsufficiency of TonEBP inhibits hepatic and hippocampal high-mobility group box-1 (HMGB1) expression in diabetic mice. Here, mice were fed a high-fat diet (HFD) for 16 weeks and received an intraperitoneal injection of 100 mg/kg streptozotocin (STZ) and followed by continued HFD feeding for an additional 4 weeks to induce hyperglycemia and hepatic steatosis. Compared with wild-type diabetic mice, diabetic TonEBP(+/-) mice showed decreased body weight, fat mass, hepatic steatosis, and macrophage infiltration. We also found that adipogenesis and HMGB1 expression in the liver and hippocampus were lower in diabetic TonEBP(+/-) mice compared with the wild type. Furthermore, iba-1 immunoreactivity in the hippocampus was decreased in diabetic TonEBP(+/-) mice compared with that in the wild type. Our findings suggest that TonEBP haploinsufficiency suppresses diabetes-associated hepatic steatosis and neuroinflammation.
provenance
Made available in Cube on 2018-09-28T16:04:34Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Jong Youl
Jeong, Eun Ae
Kim, Kyung Eun
Yi, Chin-ok
Jin, Zhen
Lee, Jung Eun
Lee, Dong Hoon
Kim, Hyun Joon
Kang, Sang Soo
Cho, Gyeong Jae
Choi, Wan Sung
Choi, Soo Youn
Kwon, H. Moo
Roh, Gu Seob
accessioned
2018-09-28T16:04:34Z
available
2018-09-28T16:04:34Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486325.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274337
Jurisdiction
Rep.of Korea
Project Name
Team for anti-aging biotechnology and industry
rights
openAccess
type
article


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