TNF-alpha-induced Inflammation Stimulates Apolipoprotein-A4 viaActivation of TNFR2 and NF-kappa B Signaling in Kidney Tubular Cells

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486312.do
DOI
10.1038/s41598-017-08785-2
Title
TNF-alpha-induced Inflammation Stimulates Apolipoprotein-A4 viaActivation of TNFR2 and NF-kappa B Signaling in Kidney Tubular Cells
Description
This study was financially supported by the "Kiturami" Faculty ResearchAssistance Program of Yonsei University College of Medicine for2014(6-2014-0151).
abstract
Apo-A4 expression was increased in tissues from chronic kidney disease (CKD) patients compared to that in normal kidney tissue. We determined the association of apo-A4 and its regulatory signals following acute kidney injury and elucidated the effects of apo-A4 on cell signaling pathways related to kidney injury in vitro and in vivo. Tumor necrosis factor (TNF)-alpha, which causes inflammatory cell injury, induced significantly increased expression of apo-A4 protein levels, and these levels were related to pro-inflammatory acute kidney injury in human kidney cells. Apo-A4 expression was also increased in experimented rat kidney tissues after ischemic reperfusion injury. The expression of tumor necrosis factor receptor (TNFR) 2 was increased in both kidney cell lines and experimented rat kidney tissues following acute kidney injury. The expression of apo-A4 and TNFR2 was increased upon treatment with TNF-alpha. Immunohistochemistry revealed positive apo-A4 and TNFR2 staining in ischemic reperfusion injury rat kidneys compared with levels in the sham operation kidneys. After neutralization of TNF-alpha, NF-kappa B expression was only observed in the cytoplasm by immunofluorescence. Therefore, the apo-A4 expression is increased by stimulation of injured kidney cells with TNF-alpha and that these effects occur via a TNFR2-NF kappa B complex.
provenance
Made available in Cube on 2018-09-28T16:04:14Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Hyung Ho
Cho, Young In
Kim, Sook Young
Yoon, Young Eun
Kim, Kyung Sup
Hong, Sung Joon
Han, Woong Kyu
accessioned
2018-09-28T16:04:14Z
available
2018-09-28T16:04:14Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486312.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274063
Jurisdiction
Rep.of Korea
Project Name
Project of Yonsei Medical Science
rights
openAccess
type
article


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