TmCactin plays an important role in Gram-negative and -positivebacterial infection by regulating expression of 7 AMP genes in Tenebriomolitor

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486308.do
DOI
10.1038/srep46459
Title
TmCactin plays an important role in Gram-negative and -positivebacterial infection by regulating expression of 7 AMP genes in Tenebriomolitor
Description
This research was supported by Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Science, ICT and future Planning (Grant No. 2015R1A2A2A01005301).
abstract
Cactin was originally identified as an interactor of the Drosophila I kappa B factor Cactus and shown to play a role in controlling embryonic polarity and regulating the NF-kappa B signaling pathway. While subsequent studies have identified the roles for Cactin in the mammalian immune response, the immune function of Cactin in insects has not been described yet. Here, we identified a Cactin gene from the mealworm beetle, Tenebrio molitor (TmCactin) and characterized its functional role in innate immunity. TmCactin was highly expressed in prepupa to last instar stages, and its expression was high in the integument and Malpighian tubules of last instar larvae and adults. TmCactin was induced in larvae after infection with different pathogens and detectable within 3 hours of infection. The highest levels of TmCactin expression were detected at 9 hours post infection. TmCactin RNAi significantly decreased the survival rates of larvae after challenge with Escherichia coli and Staphylococcus aureus, but had no significant effect after challenge with Candida albicans. Furthermore, TmCactin RNAi significantly reduced the expression of seven antimicrobial peptide genes (AMPs) after bacterial challenge. Our results suggest that TmCactin may serve as an important regulator of innate immunity, mediating AMP responses against both Gram-positive and Gram-negative bacteria in T. molitor.
provenance
Made available in Cube on 2018-09-28T16:04:07Z (GMT). No. of bitstreams: 0
language
English
author
Jo, Yong Hun
Kim, Yu Jung
Park, Ki Beom
Seong, Jeong Hwan
Kim, Soo Gon
Park, Soyi
Noh, Mi Young
Lee, Yong Seok
Han, Yeon Soo
accessioned
2018-09-28T16:04:07Z
available
2018-09-28T16:04:07Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486308.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274227
Jurisdiction
Rep.of Korea
Project Name
Global Plant Healthcare Education Team
rights
openAccess
type
article


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