Thymoquinone (TQ) inhibits the replication of intracellularMycobacterium tuberculosis in macrophages and modulates nitric oxideproduction

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486261.do
DOI
10.1186/s12906-017-1786-0
Title
Thymoquinone (TQ) inhibits the replication of intracellularMycobacterium tuberculosis in macrophages and modulates nitric oxideproduction
Description
This research was supported by a grant of the Korea Health TechnologyR&D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea(grant number: HI13C0828). This work was also supported by theSoonchunhyang University Research Fund.
abstract
Background: Human tuberculosis, which is caused by the pathogen Mycobacterium tuberculosis, remains a major public health concern. Increasing drug resistance poses a threat of disease resurgence and continues to cause considerable mortality worldwide, which necessitates the development of new drugs with improved efficacy. Thymoquinone (TQ), an essential compound of Nigella sativa, was previously reported as an active anti-tuberculosis agent.
Methods: In this study, the effects of TQ on intracellular mycobacterial replication are examined in macrophages. In addition, its effect on mycobacteria-induced NO production and pro-inflammatory responses were investigated in Mycobacterium tuberculosis (MTB)-infected Type II human alveolar and human myeloid cell lines.
Results: TQ at concentrations ranging from 12.5 to 25 mu g/mL and 6.25 to 12.5 mu g/mL reduced intracellular M. tuberculosis H37Rv and extensively drug-resistant tuberculosis (XDR-TB) 72 h post-infection in RAW 264.7 cells. TQ treatment also produced a concentration-dependent reduction in nitric oxide production in both H37Rv and XDR-TB infected RAW 264.7 cells. Furthermore, TQ reduced the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory molecules such as tumor necrosis factor-alpha (TNF-alpha) and interlukin-6 (IL-6) in H37Rv-infected cells and eventually reduced pathogen-derived stress in host cells.
Conclusions: TQ inhibits intracellular H37Rv and XDR-TB replication and MTB-induced production of NO and pro-inflammatory molecules. Therefore, along with its anti-inflammatory effects, TQ represents a prospective treatment option to combat Mycobacterium tuberculosis infection.
provenance
Made available in Cube on 2018-09-28T16:02:52Z (GMT). No. of bitstreams: 0
language
English
author
Al Mahmud, Hafij
Seo, Hoonhee
Kim, Sukyung
Islam, Md Imtiazul
Nam, Kung-Woo
Cho, Hyun-Deuk
Song, Ho-Yeon
accessioned
2018-09-28T16:02:52Z
available
2018-09-28T16:02:52Z
issued
2017
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(17)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486261.do
Funder
보건복지부
Funding Program
감염병위기대응기술개발
Project ID
1465023849
Jurisdiction
Rep.of Korea
Project Name
The effectiveness and safety research regarding anti-tuberculosis effect of natural product
rights
openAccess
subject
Mycobacterium tuberculosis
Xdr-Tb
Intracellular killing
Nitric oxide
Thymoquinone
type
article


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