The trisaccharide raffinose modulates epidermal differentiation throughactivation of liver X receptor

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486155.do
DOI
10.1038/srep43823
Title
The trisaccharide raffinose modulates epidermal differentiation throughactivation of liver X receptor
Description
This work was supported by grants from NRF-2014R1A2A1A10052265, and theBio & Medical Technology Development Program (2012M3A9B6055338).
abstract
The epidermal barrier function requires optimal keratinocyte differentiation and epidermal lipid synthesis. Liver X receptor (LXR) alpha and beta, are important transcriptional regulators of the epidermal gene expression. Here, we show that raffinose, a ubiquitously present trisaccharide in plants, activated the transcriptional activity of LXR alpha/beta, which led to the induction of genes required for keratinocyte differentiation such as involucrin and filaggrin, and genes involved in lipid metabolism and transport including SCD1 and ABCA1 in both HaCaT and normal human epidermal keratinocytes. Raffinose induced the expression of JunD and Fra1, and their DNA binding in the AP1 motif in the promoters of involucrin and loricrin. Interestingly, LXR bound the AP1 motif upon raffinose treatment, and conversely, JunD and Fra1 bound the LXR response element in promoters of LXR target genes, which indicates the presence of a postive cross-talk between LXR and AP1 in the regualtion of these genes. Finally, the effect of raffinose in epidermal barrier function was confirmed by applying raffinose in an ointment formulation to the skin of hairless mice. These findings suggest that raffinose could be examined as an ingredient in functional cosmetics and therapeutic agents for the treatment of cutaneous disorders associated with abnormal epidermal barrier function.
provenance
Made available in Cube on 2018-09-28T15:59:59Z (GMT). No. of bitstreams: 0
language
English
author
Na, Tae-Young
Kim, Gyeong-Hwan
Oh, Hyeon-Jeong
Lee, Min-Ho
Han, Yong-Hyun
Kim, Ki Taek
Kim, Ji-Su
Kim, Dae-Duk
Lee, Mi-Ock
accessioned
2018-09-28T15:59:59Z
available
2018-09-28T15:59:59Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486155.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274046
Jurisdiction
Rep.of Korea
Project Name
Research & Educational Program for Creative Global Pharmaceutical Scientists (R&E Program for CGPS)
rights
openAccess
type
article


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