The topical application of low-temperature argon plasma enhances theanti-inflammatory effect of Jaun-ointment on DNCB-induced NC/Nga mice

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486151.do
DOI
10.1186/s12906-017-1850-9
Title
The topical application of low-temperature argon plasma enhances theanti-inflammatory effect of Jaun-ointment on DNCB-induced NC/Nga mice
Description
This research was supported by a grant of the Korea Health TechnologyR&D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea(grant number: H vertical bar 15C0011). This research was also supportedby the Basic Science Research Program through the National ResearchFoundation of Korea (NRF) funded by the Ministry of Education(NRF-2014R1A1A2053390 and NRF-2016R1D1A 1A02937540).
abstract
Background: Jaun-ointment (JO), also known as Shiunko in Japan, is one of the most popular medicinal formulae used in Korean traditional medicine for the external treatment of skin wound and inflammatory skin conditions. Since JO is composed of crude mixture of two herbal extracts (radix of Lithospermum erythrorhizon Siebold & Zucc and Angelica gigas Nakai), those been proved its anti-inflammatory activities in-vitro and in-vivo, JO has been expected as a good alternative treatment option for atopic dermatitis (AD). However, due to the lack of strategies for the penetrating methods of JO's various anti-inflammatory elements into the skin, an effective and safe transdermal drug delivery system needs to be determined. Here, low-temperature argon plasma (LTAP) was adopted as an ancillary partner of topically applied JO in a mice model of AD and the effectiveness was examined.
Methods: Dorsal skins of NC/Nga mice were challenged with DNCB (2,4-dinitrochlorobenzene) to induce AD. AD-like skin lesions were treated with JO alone, or in combination with LTAP. Inflammatory activity in the skin tissues was evaluated by histological analysis and several molecular biological tests.
Results: LTAP enhanced the effect of JO on AD-like skin lesion. Topical application of JO partially inhibited the development of DNCB-induced AD, shown by the moderate reduction of eosinophil homing and pro-inflammatory cytokine level. Combined treatment of JO and LTAP dramatically inhibited AD phenotypes. Interestingly, treatment with JO alone did not affect the activity of nuclear factor (NF).B/RelA in the skin, but combined treatment of LTAP-JO blocked DCNB-mediated NF.B/RelA activation.
Conclusions: LTAP markedly enhanced the anti-inflammatory activity of JO on AD-like skin lesions. The effect of LTAP may be attributed to enhancement of drug penetration and regulation of NF.B activity. Therefore, the combination treatment of JO and LTAP could be a potential strategy for the treatment of AD.
provenance
Made available in Cube on 2018-09-28T15:59:53Z (GMT). No. of bitstreams: 0
language
English
author
Choi, Jeong-Hae
Song, Yeon-Suk
Lee, Hae-June
Kim, Gyoo-Cheon
Hong, Jin-Woo
accessioned
2018-09-28T15:59:53Z
available
2018-09-28T15:59:53Z
issued
2017
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(17)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486151.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274010
Jurisdiction
Rep.of Korea
Project Name
Creative Human Resource Development Program for IT Convergence
rights
openAccess
subject
Low-temperature argon plasma
Transdermal drug delivery of herbalextracts
Jaun-ointment
Angelica gigas Nakai
Lithospermumerythrorhizon Siebold & Zucc
DNCB-induced cutaneous inflammation inNC/Nga
type
article


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