The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridiniumIon-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formationand Mitochondrial Membrane Potential Loss

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486125.do
DOI
10.1155/2017/7982389
Title
The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridiniumIon-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formationand Mitochondrial Membrane Potential Loss
Description
This work was supported by the Mid-Career Research Program through theNational Research Foundation of Korea (NRF) grant funded by the Koreangovernment [MEST] (no. NRF-2014R1A2A1A11052795).
abstract
In a previous study, we found that the short isoform of DNAJB6 (DNAJB6(S)) had been decreased in the striatum of a mouse model of Parkinson's disease (PD) induced by 1- methyl- 4- phenyl- 1,2,3,6- tetrahydropyridine (MPTP). DNAJB6, one of the heat shock proteins, has been implicated in the pathogenesis of PD. In this study, we explored the cytoprotective effect of DNAJB6(S) against 1- methyl- 4- phenylpyridinium ion- (MPP (+)-) induced apoptosis and the underlying molecular mechanisms in cultured LN18 cells from astrocytic tumors. We observed thatMPP (+) significantly reduced the cell viability and induced apoptosis in LN18 glioblastoma cells. DNAJB6(S) protected LN18 cells against MPP (+)- induced apoptosis not only by suppressing Bax cleavage but also by inhibiting a series of apoptotic events including loss of mitochondrial membrane potential, increase in intracellular reactive oxygen species, and activation of caspase- 9. These observations suggest that the cytoprotective effects of DNAJB6(S) may be mediated, at least in part, by the mitochondrial pathway of apoptosis.
provenance
Made available in Cube on 2018-09-28T15:59:11Z (GMT). No. of bitstreams: 0
language
English
author
Hong, Yeon-Mi
Hong, Yohan
Choi, Yeong-Gon
Yeo, Sujung
Jin, Soo Hee
Lee, Sae-Won
Sung, Backil
Lee, Sook-Hyun
Jung, Hyejin
Lim, Sabina
orcid
Lim, Sabina/0000-0003-0509-6681; Lee, Seo-Yeon/0000-0002-2503-1204
accessioned
2018-09-28T15:59:11Z
available
2018-09-28T15:59:11Z
issued
2017
citation
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
issn
1942-0900
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486125.do
Funder
미래창조과학부
Funding Program
개인연구지원
Project ID
1711043146
Jurisdiction
Rep.of Korea
Project Name
Research on the Mechanisms of Genes regulated by acupuncture treatment in Parkinson’s Disease
rights
openAccess
type
article


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