The role of CREB3L4 in the proliferation of prostate cancer cells

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486091.do
DOI
10.1038/srep45300
Title
The role of CREB3L4 in the proliferation of prostate cancer cells
Description
We would like to thank Dr. KS Kim and Dr. HG Yoon for providingplasmids, antibodies and cell lines. This work was supported by theBasic Science Research Program of the National Research Foundation ofKorea (NRF) and Ministry of Education, Science and Technology (MEST),Republic or Korea (NRF-2014R1A2A2A01004396 to YHA, NRF-2013R1A1A2060537to THK) and Korea Mouse Phenotyping project (2013M3A9D5072550) ofMinistry of Science, ICT and Future Planning through the NationalResearch Foundation.
abstract
The incidence of prostate cancer (PC) is growing rapidly throughout the world, in probable association with the adoption of western style diets. Thus, understanding the molecular pathways triggering the development of PC is crucial for both its prevention and treatment. Here, we investigated the role of the metabolism-associated protein, CREB3L4, in the proliferation of PC cells. CREB3L4 was upregulated by the synthetic androgen, R1881, in LNCaP PC cells (an androgen-dependent cell line). Knockdown of CREB3L4 resulted in decreased androgen-dependent PC cell growth. LNCaP cells transfected with siCREB3L4 underwent G2/M arrest, with upregulation of the proteins cyclin B1, phospho-CDK1, p21Waf1/Cip1, and INCA1, and downregulation of cyclin D1. Moreover, depletion of CREB3L4 resulted in significantly decreased expression of a subset of androgen-receptor (AR) target genes, including PSA, FKBP5, HPGD, KLK2, and KLK4. We also demonstrated that CREB3L4 directly interacts with the AR, and increases the binding of AR to androgen response elements (AREs). We also identified a role for the unfolded protein response (and its surrogate, IRE1 alpha), in activating CREB3L4. Cumulatively, we postulate that CREB3L4 expression is mediated by an AR-IRE1 alpha axis, but is also directly regulated by AR-to-ARE binding. Thus, our study demonstrates that CREB3L4 plays a key role in PC cell proliferation, which is promoted by both AR and IRE1 alpha.
provenance
Made available in Cube on 2018-09-28T15:58:18Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Tae-Hyun
Park, Joo-Man
Kim, Mi-Young
Ahn, Yong-Ho
accessioned
2018-09-28T15:58:18Z
available
2018-09-28T15:58:18Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486091.do
Funder
과학기술정보통신부
Funding Program
바이오.의료기술개발
Project ID
1711059811
Jurisdiction
Rep.of Korea
Project Name
Establishment and service-providing of Mouse metabolic and exercise phenotyping
rights
openAccess
type
article


Files in This Item

There are no attached files.