The rice OsNAC6 transcription factor orchestrates multiple molecularmechanisms involving root structural adaptions and nicotianaminebiosynthesis for drought tolerance

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486087.do
DOI
10.1111/pbi.12673
Title
The rice OsNAC6 transcription factor orchestrates multiple molecularmechanisms involving root structural adaptions and nicotianaminebiosynthesis for drought tolerance
Description
We thank the Kyungpook National University for providing rice paddyfields and G. An (Kyung Hee University) for providing the OsNAS2-D1 andOsNAS3-D1 mutants. This research was supported by the Rural DevelopmentAdministration under the Next-Generation BioGreen 21 Program(PJ011829012016) and by the Basic Science Research Program through theNational Research Foundation of Korea (NRF-2014R1A2A1A11051690,NRF-2014R1A6A3A04053795 and NRF-2013R1A6A3A04060627).
abstract
Drought has a serious impact on agriculture worldwide. A plant's ability to adapt to rhizosphere drought stress requires reprogramming of root growth and development. Although physiological studies have documented the root adaption for tolerance to the drought stress, underlying molecular mechanisms is still incomplete, which is essential for crop engineering. Here, we identified OsNAC6-mediated root structural adaptations, including increased root number and root diameter, which enhanced drought tolerance. Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were nontransgenic controls. Genome-wide analyses of loss- and gain-of-function mutants revealed that OsNAC6 up-regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3'-phophoadenosine 5'-phosphosulphate accumulation and glycosylation, which represent multiple drought tolerance pathways. Moreover, overexpression of NICOTIANAMINE SYNTHASE genes, direct targets of OsNAC6, promoted the accumulation of the metal chelator NA and, consequently, drought tolerance. Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high-yielding crops under water-limiting conditions.
provenance
Made available in Cube on 2018-09-28T15:58:11Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Dong-Keun
Chung, Pil Joong
Jeong, Jin Seo
Jang, Geupil
Bang, Seung Woon
Jung, Harin
Kim, Youn Shic
Ha, Sun-Hwa
Choi, Yang Do
Kim, Ju-Kon
accessioned
2018-09-28T15:58:11Z
available
2018-09-28T15:58:11Z
issued
2017
citation
PLANT BIOTECHNOLOGY JOURNAL(15): 6
issn
1467-7644
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486087.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274055
Jurisdiction
Rep.of Korea
Project Name
Agricultural Biotechnology Center for Innovative Future Brains
rights
openAccess
subject
biotechnology
drought
NAC transcription factor
nicotianamine
rice
root
type
article


Files in This Item

There are no attached files.