The Retinal Pigment Epithelium Is a Notch Signaling Niche in the MouseRetina

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/486085.do
DOI
10.1016/j.celrep.2017.03.040
Title
The Retinal Pigment Epithelium Is a Notch Signaling Niche in the MouseRetina
Description
We appreciate Dr. Pelle Serup for Dll1-flox mice, Dr. Friedrich Beermannfor MART1-Cre mice, and Dr. Lin Gan for Math5-Cre mice. We also thankDr. Ryoichiro Kageyama for anti-Hes1 antibody and Mi-Ryoung Song foranti-Isl1 and anti-Vsx2 antibodies. This work was supported by grants(NRF-2009-00424, NRF-2013M3C7A1056566, and NRF-2014R1A2A2A01003069)funded by the Korean National Research Foundation (NRF).
abstract
Notch signaling in neural progenitor cell is triggered by ligands expressed in adjacent cells. To identify the sources of active Notch ligands in the mouse retina, we negatively regulated Notch ligand activity in various neighbors of retinal progenitor cells (RPCs) by eliminating mindbomb E3 ubiquitin protein ligase 1 (Mib1). Mib1-deficient retinal cells failed to induce Notch activation in intra-lineage RPCs, which prematurely differentiated into neurons; however, Mib1 in post-mitotic retinal ganglion cells was not important. Interestingly, Mib1 in the retinal pigment epithelium (RPE) also contributed to Notch activation in adjacent RPCs by supporting the localization of active Notch ligands at RPE-RPC contacts. Combining this RPE-driven Notch signaling and intra-retinal Notch signaling, we propose a model in which one RPC daughter receives extra Notch signals from the RPE to become an RPC, whereas its sister cell receives only a subthreshold level of intra-retinal Notch signal and differentiates into a neuron.
provenance
Made available in Cube on 2018-09-28T15:58:08Z (GMT). No. of bitstreams: 0
language
English
author
Ha, Taejeong
Moon, Kyeong Hwan
Dai, Le
Hatakeyama, Jun
Yoon, Keejung
Park, Hee-Sae
Kong, Young-Yoon
Shimamura, Kenji
Kim, Jin Woo
orcid
KIM, JIN WOO/0000-0003-0767-1918; Yoon, Keejung/0000-0002-2361-9039
accessioned
2018-09-28T15:58:08Z
available
2018-09-28T15:58:08Z
issued
2017
citation
CELL REPORTS(19): 2
issn
2211-1247
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/486085.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273975
Jurisdiction
Rep.of Korea
Project Name
BK21 PLUS BioKAIST Initiative
rights
openAccess
type
article


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