TGF-beta 1 regulates cell fate during epithelial-mesenchymal transitionby upregulating survivin

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/485687.do
DOI
10.1038/cddis.2013.244
Title
TGF-beta 1 regulates cell fate during epithelial-mesenchymal transitionby upregulating survivin
Description
This research was supported by the Basic Science Research Programthrough the National Research Foundation of Korea (NRF) funded by theMinistry of Education, Science and Technology (2011-0013562 to JL) andby the grant (KDDF-201202-10) from the Korea Drug Development Fund.
abstract
Members of the transforming growth factor beta (TGF-beta) superfamily are multifunctional cytokines that regulate several cellular processes, including cell cycle arrest, differentiation, morphogenesis, and apoptosis. TGF-beta promotes extracellular matrix production and morphological change. Morphogenetic responses to TGF-beta include cell migration and epithelial-mesenchymal transition (EMT), which are critical during embryogenesis, development of fibrotic diseases, and the spreading of advanced carcinomas. The purpose of this study was to clarify how TGF-beta regulates the fate of retinal pigment epithelial (RPE) cells. TGF-beta 1 promoted cell cycle progression and phosphorylation of retinoblastoma protein (Rb) in ARPE-19 cells. TGF-beta 1 induced survivin expression, which in turn stabilized tubulin and Aurora B. RT-PCR and western blot analysis revealed that survivin expression increased in ARPE-19 cells following TGF-beta 1 treatment. When survivin was depleted, TGF-beta 1 induced cell cycle arrest and apoptosis and also reduced Rb phosphorylation. In conclusion, the present study shows that induction of EMT in human RPE cells upregulates survivin, leading to survivin-dependent inhibition of cell cycle arrest and apoptosis. Whether cells undergo EMT or apoptosis in response to TGF-beta 1 is dependent on their cell cycle state, and TGF-beta 1 regulates the cell cycle via survivin.
provenance
Made available in Cube on 2018-09-28T15:47:24Z (GMT). No. of bitstreams: 0
language
English
author
Lee, J.
Choi, J-H
Joo, C-K
accessioned
2018-09-28T15:47:24Z
available
2018-09-28T15:47:24Z
issued
2013
citation
CELL DEATH & DISEASE(4)
issn
2041-4889
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/485687.do
Funder
교육과학기술부
Funding Program
일반연구자지원
Project ID
1345168999
Jurisdiction
Rep.of Korea
Project Name
Research on epithelial to mesenchymal transition(EMT) and apoptosis mechanisms to development treatment for fibrosis related disease
rights
openAccess
subject
apoptosis
cell cycle
epithelial/endothelial-mesenchymal transition
human retinal pigment epithelium
survivin
transforming growthfactor-beta 1
type
article


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