Replication and packaging of Turnip yellow mosaic virus RNA containingFlock house virus RNA1 sequence

Collection with item attached
2014
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/484211.do
DOI
10.5483/BMBRep.2014.47.6.187
Title
Replication and packaging of Turnip yellow mosaic virus RNA containingFlock house virus RNA1 sequence
Description
This study was supported by the Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education, Science and Technology (NRF-2010-0021868).
abstract
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.
provenance
Made available in Cube on 2018-09-28T15:07:56Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Hui-Bae
Kim, Do-Yeong
Cho, Tae-Ju
accessioned
2018-09-28T15:07:56Z
available
2018-09-28T15:07:56Z
issued
2014
citation
BMB REPORTS(47): 6
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/484211.do
Funder
교육부
Funding Program
일반연구자지원
Project ID
1345220900
Jurisdiction
Rep.of Korea
Project Name
Characterization of Turnip yellow mosaic virus packaging and development of an RNA delivery vector
rights
openAccess
subject
Encapsidation
FHV RNA1
Flock house virus
Recombinant virus
TYMV
type
article


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