Proteome Analysis of Bovine Longissimus dorsi Muscle Associated with theMarbling Score

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483730.do
DOI
10.5713/ajas.2012.12127
Title
Proteome Analysis of Bovine Longissimus dorsi Muscle Associated with theMarbling Score
Description
This work was supported by a grant from the Next-Generation BioGreen 21Program (no. PJ0081912011), Rural Development Administration.
abstract
The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the marbling score for bovine sirloin to select potential biomarkers to improve meat quality through comparative proteomic analysis. Proteins isolated from muscle were separated by two-dimensional gel electrophoresis. After analyzing images of the stained gel, seven protein spots for the high marbling score group were identified corresponding to changes in expression that were at least two-fold compared to the low marbling score group. Four spots with increased intensities in the high marbling score group were identified as phosphoglycerate kinase 1, triosephophate isomerase, acidic ribosomal phosphoprotein PO, and capping protein (actin filament) Z-line alpha 2. Spots with decreased intensities in the high marbling score group compared to the low score group were identified as 14-3-3 epsilon, carbonic anhydrase II, and myosin light chain 1. Expression of myosin light chain 1 and carbonic anhydrase 2 was confirmed by Western blotting. Taken together, these data could help improve the economic performance of cattle and provide useful information about the underlying the function of bovine skeletal muscle.
provenance
Made available in Cube on 2018-09-28T14:54:39Z (GMT). No. of bitstreams: 0
language
English
author
Shen, Y. N.
Kim, S. H.
Yoon, D. H.
Lee, H. G.
Kang, H. S.
Seo, K. S.
accessioned
2018-09-28T14:54:39Z
available
2018-09-28T14:54:39Z
issued
2012
citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(25): 8
issn
1011-2367
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483730.do
Funder
농촌진흥청
Funding Program
차세대바이오그린21
Project ID
1395029010
Jurisdiction
Rep.of Korea
Project Name
Development of omics technologies for high effective improvement in Hanwoo
rights
openAccess
subject
Sirloin
Skeletal Muscle
Marbling Score
Proteomics
Cattle
type
article


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