Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483720.do
DOI
10.5713/ajas.2012.12294
Title
Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows
Description
This study was supported by the Rural Development Administration (RDA)project number PJ006702.
abstract
Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment I, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.
provenance
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language
English
author
Chung, H. J.
Kim, K. W.
Han, D. W.
Lee, H. C.
Yang, B. C.
Chung, H. K.
Shim, M. R.
Choi, M. S.
Jo, E. B.
Jo, Y. M.
Oh, M. Y.
Jo, S. J.
Hong, S. K.
Park, J. K.
Chang, W. K.
accessioned
2018-09-28T14:54:22Z
available
2018-09-28T14:54:22Z
issued
2012
citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(25): 11
issn
1011-2367
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483720.do
Funder
농촌진흥청
Funding Program
축산시험연구(R&D,책임운영)
Project ID
1395028725
Jurisdiction
Rep.of Korea
Project Name
Development of transgenic animal bio-reactor producing therapeutic proteins
rights
openAccess
subject
Corpus Luteum
Korean Native Cows
Pregnancy
Progesterone
ProteomeAnalysis
type
article


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