Protein phosphorylation on tyrosine restores expression andglycosylation of cyclooxygenase-2 by 2-deoxy-D-glucose-causedendoplasmic reticulum stress in rabbit articular chondrocyte

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483719.do
DOI
10.5483/BMBRep.2012.45.5.317
Title
Protein phosphorylation on tyrosine restores expression andglycosylation of cyclooxygenase-2 by 2-deoxy-D-glucose-causedendoplasmic reticulum stress in rabbit articular chondrocyte
Description
This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea Government (MEST)(2011-0027473 &2012-0004359).
abstract
2-deoxy-D-glucose(2DG)-caused endoplasmic reticulum (ER) stress inhibits protein phosphorylation at tyrosine residues. However, the accurate regulatory mechanisms, which determine the inflammatory response of chondrocytes to ER stress via protein tyrosine phosphorylation, have not been systematically evaluated. Thus, in this study, we examined whether protein phosphorylation at tyrosine residues can modulate the expression and glycosylation of COX-2, which is reduced by 2DG-induced ER stress. We observed that protein tyrosine phosphatase (PTP) inhibitors, sodium orthovanadate (SOV), and phenylarsine oxide (PAO) significantly decreased expression of ER stress inducible proteins, glucose-regulated protein 94 (GRP94), and CCAAT/ enhancer-binding-protein- related gene (GADD153), which was induced by 2DG. In addition, we demonstrated that SOV and PAO noticeably restored the expression and glycosylalion of COX-2 after treatment with 2DG. These results suggest that protein phosphorylation of tyrosine residues plays an important role in the regulation of expression and glycosylation during 2DG-induced ER stress in rabbit articular chondrocytes. [BMB Reports 2012; 45(5): 317-322]
provenance
Made available in Cube on 2018-09-28T14:54:21Z (GMT). No. of bitstreams: 0
language
English
author
Yu, Seon-Mi
Kim, Song-Ja
accessioned
2018-09-28T14:54:21Z
available
2018-09-28T14:54:21Z
issued
2012
citation
BMB REPORTS(45): 5
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483719.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196561
Jurisdiction
Rep.of Korea
Project Name
Field Oriented Biotech HRD Project Team
rights
openAccess
subject
Chondrocyte
Cyclooxygenase-2
Glycosylation
Tyrosine phosphorylation
2-deoxy-D-glucose
type
article


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