Protein Disulfide Isomerase-Like Protein 1-1 Controls EndospermDevelopment through Regulation of the Amount and Composition of SeedProteins in Rice

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483713.do
DOI
10.1371/journal.pone.0044493
Title
Protein Disulfide Isomerase-Like Protein 1-1 Controls EndospermDevelopment through Regulation of the Amount and Composition of SeedProteins in Rice
Description
This work was supported by a grant from the Next-Generation BioGreen 21Program (Plant Molecular Breeding Center no. PJ008123), RuralDevelopment Administration, Republic of Korea. The funders had no rolein study design, data collection and analysis, decision to publish, orpreparation of the manuscript.
abstract
Protein disulfide isomerase (PDI) is a chaperone protein involved in oxidative protein folding by acting as a catalyst and assisting folding in the endoplasmic reticulum (ER). A genome database search showed that rice contains 19 PDI-like genes. However, their functions are not clearly identified. This paper shows possible functions of rice PDI-like protein 1-1 (PDIL1-1) during seed development. Seeds of the T-DNA insertion PDIL1-1 mutant, PDIL1-1D, identified by genomic DNA PCR and western blot analysis, display a chalky phenotype and a thick aleurone layer. Protein content per seed was significantly lower and free sugar content higher in PDIL1-1D mutant seeds than in the wild type. Proteomic analysis of PDIL1-1D mutant seeds showed that PDIL1-1 is post-translationally regulated, and its loss causes accumulation of many types of seed proteins including glucose/starch metabolism-and ROS (reactive oxygen species) scavenging-related proteins. In addition, PDIL1-1 strongly interacts with the cysteine protease OsCP1. Our data indicate that the opaque phenotype of PDIL1-1D mutant seeds results from production of irregular starch granules and protein body through loss of regulatory activity for various proteins involved in the synthesis of seed components.
provenance
Made available in Cube on 2018-09-28T14:54:11Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Yeon Jeong
Yeu, Song Yion
Park, Bong Soo
Koh, Hee-Jong
Song, Jong Tae
Seo, Hak Soo
accessioned
2018-09-28T14:54:11Z
available
2018-09-28T14:54:11Z
issued
2012
citation
PLOS ONE(7): 9
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483713.do
Funder
농촌진흥청
Funding Program
차세대바이오그린21
Project ID
1395025433
Jurisdiction
Rep.of Korea
Project Name
Study of rice seed development by post-translational modification
rights
openAccess
type
article


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