Protective effect of the methanol extract from Cryptotaenia japonicaHassk. against lipopolysaccharide-induced inflammation in vitro and invivo

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483700.do
DOI
10.1186/1472-6882-12-199
Title
Protective effect of the methanol extract from Cryptotaenia japonicaHassk. against lipopolysaccharide-induced inflammation in vitro and invivo
abstract
Background: In folk medicine, the aerial part of Crytotaenia japonica Hassk. (CJ), is applied for treatment of the common cold, cough, urinary problems, pneumonia, and skin rashes. In this paper, the in vitro and in vivo anti-inflammatory activity of CJ methanol extract was tested using lipopolysaccharide (LPS)-induced inflammatory models.
Methods: We measured nitric oxide (NO), inducible NO synthase (iNOS), and inflammatory cytokine levels from LPS-stimulated mouse peritoneal macrophages. Also, several cellular signaling molecules which regulate the expressions of these inflammatory markers were examined. Finally, we tested whether oral administration of CJ methanol extract might affect the serum cytokine levels in LPS-injected mice.
Results: CJ methanol extract reduced NO release via iNOS protein inhibition. The extract was also shown to decrease the secretions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-12. Analysis of signaling molecules showed that CJ inhibited the phosphorylation of STAT1, p38, JNK and ERK1/2 as well as IkBa degradation. Finally, CJ decreased the serum levels of TNF-alpha and IL-6 in LPS-injected mice.
Conclusions: Our results demonstrated the anti-inflammatory activity of CJ methanol extract and its possible underlying mechanisms that involve modulation of IkBa, MAPK, and STAT1 activities.
provenance
Made available in Cube on 2018-09-28T14:53:50Z (GMT). No. of bitstreams: 0
language
English
author
Kang, Hee
Bang, Tae-Sun
Lee, Ji-Won
Lew, Jae-Hwan
Eom, Seok Hyun
Lee, Kyungjin
Choi, Ho-Young
accessioned
2018-09-28T14:53:50Z
available
2018-09-28T14:53:50Z
issued
2012
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(12)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483700.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196514
Jurisdiction
Rep.of Korea
Project Name
한의과학사업단
rights
openAccess
subject
Cryptotaenia japonica Hassk.
Inflammation
Macrophages
iNOS
Cytokines
Signaling
type
article


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