Protease-Activated Receptor 2 Mediates Mucus Secretion in the AirwaySubmucosal Gland

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483676.do
DOI
10.1371/journal.pone.0043188
Title
Protease-Activated Receptor 2 Mediates Mucus Secretion in the AirwaySubmucosal Gland
Description
This study was supported by a grant of the Korea Health TechnologyResearch & Development Project, Ministry of Health & Welfare, Republicof Korea (A110096). The funders had no role in study design, datacollection and analysis, decision to publish, or preparation of themanuscript.
abstract
Protease-activated receptor 2 (PAR2), a G protein-coupled receptor expressed in airway epithelia and smooth muscle, plays an important role in airway inflammation. In this study, we demonstrated that activation of PAR2 induces mucus secretion from the human airway gland and examined the underlying mechanism using the porcine and murine airway glands. The mucosa with underlying submucosal glands were dissected from the cartilage of tissues, pinned with the mucosal side up at the gas/bath solution interface of a physiological chamber, and covered with oil so that secretions from individual glands could be visualized as spherical bubbles in the oil. Secretion rates were determined by optical monitoring of the bubble diameter. The Ca2+-sensitive dye Fura2-AM was used to determine intracellular Ca2+ concentration ([Ca2+](i)) by means of spectrofluorometry. Stimulation of human tracheal mucosa with PAR2-activating peptide (PAR2-AP) elevated intracellular Ca2+ and induced glandular secretion equal to approximately 30% of the carbachol response in the human airway. Porcine gland tissue was more sensitive to PAR2-AP, and this response was dependent on Ca2+ and anion secretion. When the mouse trachea were exposed to PAR2-AP, large amounts of secretion were observed in both wild type and Delta F508 cystic fibrosis transmembrane conductance regulator mutant mice but there is no secretion from PAR-2 knock out mice. In conclusion, PAR2-AP is an agonist for mucus secretion from the airway gland that is Ca2+-dependent and cystic fibrosis transmembrane conductance regulator-independent.
provenance
Made available in Cube on 2018-09-28T14:53:11Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Hyun Jae
Yang, Yu-Mi
Kim, Kyubo
Shin, Dong Min
Yoon, Joo-Heon
Cho, Hyung-Ju
Choi, Jae Young
orcid
Yoon, Joo-Heon/0000-0003-2404-7156
accessioned
2018-09-28T14:53:11Z
available
2018-09-28T14:53:11Z
issued
2012
citation
PLOS ONE(7): 8
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483676.do
Funder
보건복지부
Funding Program
보건의료기술연구개발
Project ID
1465011067
Jurisdiction
Rep.of Korea
Project Name
Evaluation of the pseudomonas elastase inhibitors in airway infection model
rights
openAccess
type
article


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