Protease activated receptor 1-induced glutamate release in culturedastrocytes is mediated by Bestrophin-1 channel but not by vesicularexocytosis

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483675.do
DOI
10.1186/1756-6606-5-38
Title
Protease activated receptor 1-induced glutamate release in culturedastrocytes is mediated by Bestrophin-1 channel but not by vesicularexocytosis
Description
This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MEST) (No.314-2008-1-C00341).
abstract
Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear.
Findings: We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes.
Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.
provenance
Made available in Cube on 2018-09-28T14:53:10Z (GMT). No. of bitstreams: 0
language
English
author
Oh, Soo-Jin
Han, Kyung-Seok
Park, Hyungju
Woo, Dong Ho
Kim, Hye Yun
Traynelis, Stephen F.
Lee, C. Justin
orcid
Park, Hyungju/0000-0002-9010-9434
accessioned
2018-09-28T14:53:10Z
available
2018-09-28T14:53:10Z
issued
2012
citation
MOLECULAR BRAIN(5)
issn
1756-6606
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483675.do
Funder
교육과학기술부
Funding Program
한국과학기술연구원연구운영비지원
Project ID
1345197036
Jurisdiction
Rep.of Korea
Project Name
마음-행동의 뇌 회로 작성 및 조절 연구
rights
openAccess
subject
Astrocyte
Bestrophin-1
Glutamate
type
article


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