Prophylactic Effect of Probiotics on the Development of ExperimentalAutoimmune Myasthenia Gravis

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483662.do
DOI
10.1371/journal.pone.0052119
Title
Prophylactic Effect of Probiotics on the Development of ExperimentalAutoimmune Myasthenia Gravis
Description
This research was supported by grants from the Regional TechnologyInnovation Program of the MOCIE (RTI05-01-01), Mid-career ResearcherProgram through NRF (National Research Foundation, KOREA) (2011-0000126)and by a Systems Biology Infrastructure Establishment Grant provided byGIST (Gwangju Institute of Science and Technology). The funders had norole in study design, data collection and analysis, decision to publish,or preparation of the manuscript.
abstract
Probiotics are live bacteria that confer health benefits to the host physiology. Although protective role of probiotics have been reported in diverse diseases, no information is available whether probiotics can modulate neuromuscular immune disorders. We have recently demonstrated that IRT5 probiotics, a mixture of 5 probiotics, could suppress diverse experimental disorders in mice model. In this study we further investigated whether IRT5 probiotics could modulate the progression of experimental autoimmune myasthenia gravis (EAMG). Myasthenia gravis (MG) is a T cell dependent antibody mediated autoimmune disorder in which acetylcholine receptor (AChR) at the neuromuscular junction is the major autoantigen. Oral administration of IRT5 probiotics significantly reduced clinical symptoms of EAMG such as weight loss, body trembling and grip strength. Prophylactic effect of IRT5 probiotics on EMAG is mediated by down-regulation of effector function of AChR-reactive T cells and B cells. Administration of IRT5 probiotics decreased AChR-reactive lymphocyte proliferation, anti-AChR reactive IgG levels and inflammatory cytokine levels such as IFN-gamma, TNF-alpha, IL-6 and IL-17. Downregulation of inflammatory mediators in AChR-reactive lymphocytes by IRT5 probiotics is mediated by the generation of regulatory dendritic cells (rDCs) that express increased levels of IL-10, TGF-beta, arginase 1 and aldh1a2. Furthermore, DCs isolated from IRT5 probiotics-fed group effectively converted CD4(+) T cells into CD4+ Foxp3(+) regulatory T cells compared with control DCs. Our data suggest that IRT5 probiotics could be applicable to modulate antibody mediated autoimmune diseases including myasthenia gravis.
provenance
Made available in Cube on 2018-09-28T14:52:49Z (GMT). No. of bitstreams: 0
language
English
author
Chae, Chang-Suk
Kwon, Ho-Keun
Hwang, Ji-Sun
Kim, Jung-Eun
Im, Sin-Hyeog
orcid
Im, Sin-Hyeog/0000-0002-3173-1856
accessioned
2018-09-28T14:52:49Z
available
2018-09-28T14:52:49Z
issued
2012
citation
PLOS ONE(7): 12
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483662.do
Funder
지식경제부
Funding Program
지역전략산업육성
Project ID
1415124166
Jurisdiction
Rep.of Korea
Project Name
Clinical Vaccine R&D Center
rights
openAccess
type
article


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