Profiles of Multidrug Resistance Protein-1 in the Peripheral BloodMononuclear Cells of Patients with Refractory Epilepsy

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483600.do
DOI
10.1371/journal.pone.0036985
Title
Profiles of Multidrug Resistance Protein-1 in the Peripheral BloodMononuclear Cells of Patients with Refractory Epilepsy
Description
This study was supported by the Ministry of Health and Welfare, Republicof Korea (A090538). The funders had no role in study design, datacollection and analysis, decision to publish, or preparation of themanuscript.
abstract
Background: About one third of patients with epilepsy become refractory to therapy despite receiving adequate medical treatment, possibly from multidrug resistance. P-glycoprotein, encoded by multidrug resistance protein-1 (MDR1) gene, at the blood brain barrier is considered as a major factor mediating drug efflux and contributing to resistance. Given that peripheral blood mononuclear cells (PBMNCs) express MDR1, we investigated a MDR1 status of PBMNCs in various subsets of epilepsy patients and demonstrated their association with clinical characteristics.
Methodology/Principal Findings: Clinical and MDR1 data were collected from 140 patients with epilepsy, 30 healthy volunteers, and 20 control patients taking anti-epileptic drugs. PBMNCs were isolated, and basal MDR1 levels and MDR1 conformational change levels were measured by flow cytometry. MDR1 profiles were analyzed according to various clinical parameters, including seizure frequency and number of medications used in epilepsy patients. Epilepsy patients had a higher basal MDR1 level than non-epilepsy groups (p < 0.01). Among epilepsy patients, there is a tendency for higher seizure frequency group to have higher basal MDR1 level (p = 0.059). The MDR1 conformational change level was significantly higher in the high-medication-use group than the low-use group (p = 0.028). Basal MDR1 (OR = 1.16 [95% CI: 1.060-1.268]) and conformational change level (OR = 1.11 [95% CI: 1.02-1.20]) were independent predictors for seizure frequency and number of medications, respectively.
Conclusions/Significance: The MDR1 profile of PBMNCs is associated with seizure frequency and medication conditions in patients with epilepsy.
provenance
Made available in Cube on 2018-09-28T14:51:09Z (GMT). No. of bitstreams: 0
language
English
author
Ban, Jae-Jun
Jung, Keun-Hwa
Chu, Kon
Lee, Soon-Tae
Jeon, Daejong
Park, Kyung-Il
Moon, Hye-Jin
Kim, Hyeyun
Kim, Sunghun
Lee, Sang Kun
Roh, Jae-Kyu
orcid
Hess, Christopher/0000-0002-5132-5302
accessioned
2018-09-28T14:51:09Z
available
2018-09-28T14:51:09Z
issued
2012
citation
PLOS ONE(7): 5
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483600.do
Funder
보건복지부
Funding Program
보건의료기술연구개발
Project ID
1355061332
Jurisdiction
Rep.of Korea
Project Name
Transcriptome analysis and therapeutic development for epileptogenesis and intractable epilepsy
rights
openAccess
type
article


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