Production of taxadiene from cultured ginseng roots transformed withtaxadiene synthase gene

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483597.do
DOI
10.5483/BMBRep.2012.45.10.085
Title
Production of taxadiene from cultured ginseng roots transformed withtaxadiene synthase gene
Description
This work was supported by a grant No. 331-2008-1-F00019 from the BasicScience Research Program through the NRF and by a grant No. PJ008034from Systems and Synthetic Agro-biotech Center through Next-GenerationBioGreen 21 Program, RDA, Korea. Our group appreciates Dr. RodneyCroteau for the kind distribution of a cloned taxadiene synthase gene.
abstract
Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 mu g taxadiene per gram cf dry weight In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 mu g per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene. [BMB Reports 2012; 45(10): 589-594]
provenance
Made available in Cube on 2018-09-28T14:51:04Z (GMT). No. of bitstreams: 0
language
English
author
Cha, Mijeong
Shim, Sang Hee
Kim, Sung Hong
Kim, Ok Tae
Lee, Se-Weon
Kwon, Suk-Yoon
Baek, Kwang-Hyun
accessioned
2018-09-28T14:51:04Z
available
2018-09-28T14:51:04Z
issued
2012
citation
BMB REPORTS(45): 10
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483597.do
Funder
교육과학기술부
Funding Program
한국기초과학지원연구원연구운영비지원
Project ID
1345194944
Jurisdiction
Rep.of Korea
Project Name
A Study on Management of Daegu Center
rights
openAccess
subject
Panax ginseng CA Meyer
Root culture
Taxadiene synthase
Taxol
Transformation
type
article


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