Preventive Effects of Epigallocatechin-3-O-Gallate against ReplicativeSenescence Associated with p53 Acetylation in Human Dermal Fibroblasts

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/483554.do
DOI
10.1155/2012/850684
Title
Preventive Effects of Epigallocatechin-3-O-Gallate against ReplicativeSenescence Associated with p53 Acetylation in Human Dermal Fibroblasts
Description
This work was partly supported by a Grant from the Fundamental R&DProgram for Core Technology of Materials funded by the Ministry ofKnowledge Economy, Republic of Korea (K0006028) and by Basic ScienceResearch Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education, Science, and Technology(2012-0003645).
abstract
Considering the various pharmacological activities of epigallocatechin-3-O-gallate (EGCG) including anticancer, and anti-inflammatory, antidiabetic, and so forth, relatively less attention has been paid to the antiaging effect of EGCG on primary cells. In this study, the preventive effects of EGCG against serial passage-induced senescence were investigated in primary cells including rat vascular smooth muscle cells (RVSMCs), human dermal fibroblasts (HDFs), and human articular chondrocytes (HACs). The involvement of Sirt1 and acetylated p53 was examined as an underlying mechanism for the senescence preventive activity of EGCG in HDFs. All cells were employed with the initial passage number (PN) between 3 and 7. For inducing senescence, the cells were serially passaged at the predetermined times and intervals in the absence or presence of EGCG (50 or 100 mu M). Serial passage-induced senescence in RVSMCs and HACs was able to be significantly prevented at 50 mu M EGCG, while in HDFs, 100 mu M EGCG could significantly prevent senescence and recover their cell cycle progression close to the normal level. Furthermore, EGCG was found to prevent serial passage- and H2O2-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected. In addition, proliferating HDFs showed similar cellular uptake of FITC-conjugated EGCG into the cytoplasm with their senescent counterparts but different nuclear translocation of it from them, which would partly account for the differential responses to EGCG in proliferating versus senescent cells. Taking these results into consideration, it is suggested that EGCG may be exploited to craft strategies for the development of an antiaging or age-delaying agent.
provenance
Made available in Cube on 2018-09-28T14:49:56Z (GMT). No. of bitstreams: 0
language
English
author
Han, Dong-Wook
Lee, Mi Hee
Kim, Bongju
Lee, Jun Jae
Hyon, Suong-Hyu
Park, Jong-Chul
orcid
Han, Dong-Wook/0000-0001-8314-1981; Kim, Bongju/0000-0001-7309-5977
accessioned
2018-09-28T14:49:56Z
available
2018-09-28T14:49:56Z
issued
2012
citation
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
issn
1942-0900
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/483554.do
Funder
지식경제부
Funding Program
부품소재산업경쟁력향상(소재부품기술개발)
Project ID
1415122027
Jurisdiction
Rep.of Korea
Project Name
stent material and manufacturing technology
rights
openAccess
type
article


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