Nuclear Translocation of Acinetobacter baumannii Transposase Induces DNAMethylation of CpG Regions in the Promoters of E-cadherin Gene

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/482557.do
DOI
10.1371/journal.pone.0038974
Title
Nuclear Translocation of Acinetobacter baumannii Transposase Induces DNAMethylation of CpG Regions in the Promoters of E-cadherin Gene
Description
This research was supported by Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education, Science and Technology (2010-0016456). The funders had norole in study design, data collection and analysis, decision to publish,or preparation of the manuscript.
abstract
Nuclear targeting of bacterial proteins has emerged as a pathogenic mechanism whereby bacterial proteins induce host cell pathology. In this study, we examined nuclear targeting of Acinetobacter baumannii transposase (Tnp) and subsequent epigenetic changes in host cells. Tnp of A. baumannii ATCC 17978 possesses nuclear localization signals (NLSs), 225RKRKRK230. Transient expression of A. baumannii Tnp fused with green fluorescent protein (GFP) resulted in the nuclear localization of these proteins in COS-7 cells, whereas the truncated Tnp without NLSs fused with GFP were exclusively localized in the cytoplasm. A. baumannii Tnp was found in outer membrane vesicles, which delivered this protein to the nucleus of host cells. Nuclear expression of A. baumannii Tnp fused with GFP in A549 cells induced DNA methylation of CpG regions in the promoters of E-cadherin (CDH1) gene, whereas the cytoplasmic localization of the truncated Tnp without NLSs fused with GFP did not induce DNA methylation. DNA methylation in the promoters of E-cadherin gene induced by nuclear targeting of A. baumannii Tnp resulted in down-regulation of gene expression. In conclusion, our data show that nuclear traffic of A. baumannii Tnp induces DNA methylation of CpG regions in the promoters of E-cadherin gene, which subsequently down-regulates gene expression. This study provides a new insight into the epigenetic control of host genes by bacterial proteins.
provenance
Made available in Cube on 2018-09-28T14:23:07Z (GMT). No. of bitstreams: 0
language
English
author
Moon, Dong Chan
Choi, Chul Hee
Lee, Su Man
Lee, Jung Hwa
Kim, Seung Il
Kim, Dong Sun
Lee, Je Chul
accessioned
2018-09-28T14:23:07Z
available
2018-09-28T14:23:07Z
issued
2012
citation
PLOS ONE(7): 6
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/482557.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196499
Jurisdiction
Rep.of Korea
Project Name
KNU Medical Education Program for Human Resources
rights
openAccess
type
article


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